Jansen G, Deddens B, Wilkinson M, van der Waaij D
Laboratory of Medical Microbiology, University of Groningen, The Netherlands.
Infection. 1995 Jan-Feb;23(1):46-50. doi: 10.1007/BF01710058.
The influence of Enterococcus faecalis on the morphology of the bacterial cells which make up the gut microflora and on the levels of circulating IgG bound to the gut microflora was assessed. After 29 days of pretreatment monitoring, ten healthy human volunteers ingested 10(7) viable cells of E. faecalis three times daily, for 21 days. After this treatment another 21 days of follow-up completed the study. Each volunteer delivered eleven faecal samples during the entire study period of 71 days with a 7 day interval. Before and after the faeces sampling period, blood samples were collected from all volunteers. The influence of the ingestion of E. faecalis on the morphology of the gut microflora was measured by image analysis. In addition, the binding of circulating IgG to intestinal bacteria in all intermediate faecal samples was measured by means of quantitative immunofluorescence. The oral administration of E. faecalis resulted in a significant change of the morphological composition of the gut microflora and in a significant decrease in IgG-binding capacity of the gut microflora.
评估了粪肠球菌对构成肠道微生物群的细菌细胞形态以及与肠道微生物群结合的循环IgG水平的影响。在进行29天的预处理监测后，10名健康人类志愿者每天三次摄入10⁷个粪肠球菌活细胞，持续21天。该治疗后再进行21天的随访以完成研究。在整个71天的研究期间，每位志愿者每隔7天提供11份粪便样本。在粪便采样期前后，采集所有志愿者的血液样本。通过图像分析测量摄入粪肠球菌对肠道微生物群形态的影响。此外，通过定量免疫荧光测量所有中间粪便样本中循环IgG与肠道细菌的结合。口服粪肠球菌导致肠道微生物群的形态组成发生显著变化，并且肠道微生物群的IgG结合能力显著降低。
