使用改良的空斑试验技术对实体器官移植受者群体中的巨细胞病毒血症进行定量分析。
Use of a modified shell vial technique to quantitate cytomegalovirus viremia in a population of solid-organ transplant recipients.
作者信息
Buller R S, Bailey T C, Ettinger N A, Keener M, Langlois T, Miller J P, Storch G A
机构信息
Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri 63110.
出版信息
J Clin Microbiol. 1992 Oct;30(10):2620-4. doi: 10.1128/jcm.30.10.2620-2624.1992.
Abstract
A quantitative modification of the shell vial assay was used to investigate cytomegalovirus viremia in solid-organ transplant recipients. The level of viremia detected in 109 of 407 specimens ranged from 0.02 to 28 infectious foci per 100,000 leukocytes. By using a Poisson model, a technique was developed to determine 95% confidence limits for the measured levels of viremia. These confidence limits were used to determine the level of viremia that could be excluded by culturing a given number of cells. Longitudinal assessment of two transplant recipients revealed different patterns of viremia and demonstrated that significant disease sometimes occurred with low-level viremia. On the basis of the results of the studies, culture of at least 4 x 10(6) leukocytes is recommended for the sensitive detection of cytomegalovirus viremia.
摘要
采用空斑试验的定量改良方法来研究实体器官移植受者的巨细胞病毒血症。在407份标本中的109份检测到的病毒血症水平为每100,000个白细胞中有0.02至28个感染灶。通过使用泊松模型，开发了一种技术来确定所测病毒血症水平的95%置信限。这些置信限用于确定通过培养一定数量的细胞可排除的病毒血症水平。对两名移植受者的纵向评估揭示了不同的病毒血症模式，并表明低水平病毒血症有时会发生严重疾病。根据研究结果，建议培养至少4×10⁶个白细胞以灵敏检测巨细胞病毒血症。
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