Becker F F, Wang X B, Huang Y, Pethig R, Vykoukal J, Gascoyne P R
Department of Molecular Pathology, University of Texas MD Anderson Cancer Center, Houston 77030.
Proc Natl Acad Sci U S A. 1995 Jan 31;92(3):860-4. doi: 10.1073/pnas.92.3.860.
Electrorotation measurements were used to demonstrate that the dielectric properties of the metastatic human breast cancer cell line MDA231 were significantly different from those of erythrocytes and T lymphocytes. These dielectric differences were exploited to separate the cancer cells from normal blood cells by appropriately balancing the hydrodynamic and dielectrophoretic forces acting on the cells within a dielectric affinity column containing a microelectrode array. The operational criteria for successful particle separation in such a column are analyzed and our findings indicate that the dielectric affinity technique may prove useful in a wide variety of cell separation and characterization applications.
旋转电泳测量被用于证明转移性人类乳腺癌细胞系MDA231的介电特性与红细胞和T淋巴细胞的介电特性显著不同。通过在含有微电极阵列的介电亲和柱内适当平衡作用于细胞的流体动力和介电泳力，利用这些介电差异将癌细胞与正常血细胞分离。分析了在此类柱中成功进行颗粒分离的操作标准，我们的研究结果表明，介电亲和技术可能在各种细胞分离和表征应用中证明是有用的。
