两种检测伯氏疏螺旋体特异性抗体鞘内合成方法与用于莱姆病神经伯氏疏螺旋体病诊断的PCR的比较。
Comparison of two methods for detecting intrathecal synthesis of Borrelia burgdorferi-specific antibodies and PCR for diagnosis of Lyme neuroborreliosis.
作者信息
Zbinden R, Goldenberger D, Lucchini G M, Altwegg M
机构信息
Institute of Microbiology, Kantonsspital, Aarau, Switzerland.
出版信息
J Clin Microbiol. 1994 Jul;32(7):1795-8. doi: 10.1128/jcm.32.7.1795-1798.1994.
Abstract
An indirect immunofluorescence assay (IFA), a commercial capture enzyme-linked immunosorbent assay (ELISA), and PCR were compared for the diagnosis of Lyme neuroborreliosis in 16 individuals. Borrelia burgdorferi-specific intrathecal immunoglobulin G was found in 10 of 16 patients by IFA and in 8 of 12 patients by capture ELISA. PCRs performed on cerebrospinal fluid samples stored at -70 degrees C were positive for one of eight children and one of four adults with intrathecal antibody production and negative for all four adults without intrathecal antibody production. For two children with facial palsy following erythema chronicum migrans, neither IFA nor capture ELISA detected specific intrathecal antibodies and PCR was also negative. We conclude that both clinical manifestations and laboratory parameters are important for the diagnosis of Lyme neuroborreliosis.
摘要
对16名个体采用间接免疫荧光法（IFA）、商用捕获酶联免疫吸附测定法（ELISA）和聚合酶链反应（PCR）来诊断莱姆病神经疏螺旋体病。通过IFA在16例患者中的10例检测到伯氏疏螺旋体特异性鞘内免疫球蛋白G，通过捕获ELISA在12例患者中的8例检测到。对储存在-70℃的脑脊液样本进行的PCR检测显示，8名儿童中有1名、4名有鞘内抗体产生的成人中有1名呈阳性，而4名无鞘内抗体产生的成人全部呈阴性。对于2例在慢性游走性红斑后出现面瘫的儿童，IFA和捕获ELISA均未检测到特异性鞘内抗体，PCR检测也为阴性。我们得出结论，临床表现和实验室参数对莱姆病神经疏螺旋体病的诊断均很重要。
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