Zimmermann W, Breter H, Rudolph M, Ludwig H
Institut für Virologie, Freie Universität Berlin, Berlin, Federal Republic of Germany.
J Virol. 1994 Oct;68(10):6755-8. doi: 10.1128/JVI.68.10.6755-6758.1994.
The etiological agent of Borna disease, a persistent virus infection of the central nervous system with differently expressed symptomatology, was morphologically unknown. Here we provide the first convincing data on its phenotypic architecture. Salt-released virus comprising the biological parameters of Koch's postulates has an unsegmented single-stranded RNA. A dense band (1.22 g/cm3) in CsCl contains 90-nm particles which appear to be enveloped and a fraction of 50- to 60-nm particles. Labeling of the virions with neutralizing antisera and colloidal gold conjugates indicates that the 90-nm particles most likely represent the causative agent.
博尔纳病是一种中枢神经系统的持续性病毒感染，具有不同表现的症状，其病原体在形态学上尚不明确。在此，我们提供了关于其表型结构的首个确凿数据。符合科赫法则生物学参数的盐释放病毒具有一条未分段的单链RNA。氯化铯中的一条致密带（1.22 g/cm³）含有90纳米的颗粒，这些颗粒似乎有包膜，还有一部分50至60纳米的颗粒。用中和抗血清和胶体金偶联物对病毒粒子进行标记表明，90纳米的颗粒很可能代表病原体。
