等电聚焦揭示的小鼠免疫球蛋白轻链的遗传多态性
Genetic polymorphism of mouse immunoglobulin light chains revealed by isoelectric focusing.
作者信息
Gibson D
出版信息
J Exp Med. 1976 Jul 1;144(1):298-303. doi: 10.1084/jem.144.1.298.
Abstract
Light chains isolated from normal immunoglobulin of unimmunized mice were analyzed by gel isoelectric focusing. Examination of the focusing patterns of light chains from nine inbred mouse strains showed that six of the strains (SWR/J, C3H/HeJ, DBA/1J, A/J, CBA/J, and C57BL/6J) possessed a virtually identical spectrum of focusing bands, while the remaining three strains (RF/J, AKR/J, and C58/J) showed clear differences involving several bands. Analysis of the light chains of individual SWR/J, C58/J, and F1 hybrid mice indicated that the differences in focusing pattern were inherited in a simple codominant fashion. A new procedure was developed for the rapid analysis of light chains from small quantities of serum.
摘要
通过凝胶等电聚焦分析从未免疫小鼠的正常免疫球蛋白中分离出的轻链。对九个近交系小鼠品系的轻链聚焦模式进行检查发现，其中六个品系（SWR/J、C3H/HeJ、DBA/1J、A/J、CBA/J和C57BL/6J）具有几乎相同的聚焦带谱，而其余三个品系（RF/J、AKR/J和C58/J）则显示出涉及几条带的明显差异。对个体SWR/J、C58/J和F1杂种小鼠的轻链分析表明，聚焦模式的差异是以简单共显性方式遗传的。开发了一种新方法用于快速分析少量血清中的轻链。
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