Geschwender H H, Rutter G, Lehmann-Grube F
Med Microbiol Immunol. 1976 Jun 1;162(2):119-31. doi: 10.1007/BF02121321.
Lymphocytic choriomeningitis (LCM) virus-specific complement-fixing (CF) antigen (ECFA) has been solubilized, concentrated, and partially purified. When inoculated together with Freund's adjuvant, ECFA induced CF antibody but not neutralizing antibody or protective immunity. By itself it boosted pre-existing CF antibody but no neutralizing antibody. In double diffusion tests one line developed between ECFA and its antiserum, and a corresponding line became visible when ECFA interacted with an antiserum directed against all LCM virus-specific antigens. Absorption of either serum with ECFA abolished all ECFA-precipitating qualities. Ouchterlony tests also revealed that ECFA prepared from cells and tissues of various species is immunologically identical. By a variety of procedures ECFA was not found to be represented on the surface of either the virion or the infected cell. When purified infectious LCM virus was disrupted, a CF antigen corresponding immunologically to ECFA was set free. In double diffusion tests this antigen gave a line of identity with ECFA. Thus, ECFA appears to be an internal component of the infectious LCM virus.
淋巴细胞性脉络丛脑膜炎（LCM）病毒特异性补体结合（CF）抗原（ECFA）已被溶解、浓缩并部分纯化。当与弗氏佐剂一起接种时，ECFA诱导产生CF抗体，但不诱导中和抗体或保护性免疫。其自身可增强预先存在的CF抗体，但不增强中和抗体。在双向扩散试验中，ECFA与其抗血清之间形成一条沉淀线，当ECFA与针对所有LCM病毒特异性抗原的抗血清相互作用时，可见一条相应的沉淀线。用ECFA吸收任何一种血清都会消除所有ECFA的沉淀特性。欧氏免疫扩散试验还表明，从不同物种的细胞和组织中制备的ECFA在免疫学上是相同的。通过各种方法未发现ECFA存在于病毒粒子或受感染细胞的表面。当纯化的感染性LCM病毒被破坏时，释放出一种在免疫学上与ECFA相对应的CF抗原。在双向扩散试验中，这种抗原与ECFA形成一条同一沉淀线。因此，ECFA似乎是感染性LCM病毒的一种内部成分。
