Harding S A, Sandford G R, Merz W G
Am J Clin Pathol. 1976 Jun;65(6):1001-9. doi: 10.1093/ajcp/65.6.1001.
One hundred fifty-one sera from 100 hospitalized patients with positive cultures for yeasts were assayed using whole-cell agglutination (Aggl.), agar gel diffusion (AGD), and counterimmunoelectrophoresis (CEP) to determine the relative diagnostic values of three serologic tests for anti-Candida antibodies. Serial samples were obtained from 29 patients. Tests were read blindly; correlations of the three test results with culture results and clinical findings were determined only after all data had been accumulated. Thirty-five of 100 patients had Aggl. titers of 1:160 or greater, although 13/35 had no evidence of deep or disseminated disease. Twenty-four of 100 patients had clinical or autopsy evidence of deep or disseminated candidiasis; 22/24 had Aggl. titers of 1:160 or greater. Twenty of the 24 patients were CEP-positive, whereas 18/24 were AGD-positive. In five patients CEP became positive earlier (10--21 days) than AGD. Three patients had false-positive precipitin tests, two by both CEP and AGD and the third by CEP only. In this population, a positive CEP and a positive AGD test showed good correlation with deep or disseminated candidiasis, whereas a negatvie Aggl. test showed the best correlation for excluding deep or systemic candidiasis.
对100例酵母培养阳性的住院患者的151份血清进行了全细胞凝集试验（Aggl.）、琼脂凝胶扩散试验（AGD）和对流免疫电泳试验（CEP），以确定这三种抗念珠菌抗体血清学检测的相对诊断价值。从29例患者中获取了系列样本。试验结果由专人在不知情的情况下判读；仅在所有数据积累完成后，才确定三种检测结果与培养结果及临床发现之间的相关性。100例患者中有35例Aggl.效价为1:160或更高，尽管其中13/35例没有深部或播散性疾病的证据。100例患者中有24例有深部或播散性念珠菌病的临床或尸检证据；22/24例Aggl.效价为1:160或更高。24例患者中有20例CEP检测呈阳性，而18/24例AGD检测呈阳性。在5例患者中，CEP比AGD更早呈阳性（10 - 21天）。3例患者的沉淀素试验出现假阳性，2例同时在CEP和AGD检测中出现，第3例仅在CEP检测中出现。在这组人群中，CEP阳性和AGD阳性检测结果与深部或播散性念珠菌病有良好的相关性，而Aggl.检测阴性对于排除深部或系统性念珠菌病的相关性最佳。
