Haller M, Akbulut C, Brechtelsbauer H, Fett W, Briegel J, Finsterer U, Peter K
Department of Anesthesiology, Ludwig-Maximilians-University, München, Federal Republic of Germany.
Life Sci. 1993;53(21):1597-604. doi: 10.1016/0024-3205(93)90183-4.
We investigated the feasibility of using indocyanine green (ICG) for plasma volume (PV) determination in man. Duplicate PV measurements were carried out in 23 healthy subjects to test repeatability. ICG (0.25 mg/kg) was injected intravenously into one arm and venous blood was withdrawn from the opposite arm. Optical density of plasma samples from minute 3 to 9 was measured in a densitometer. ICG concentration at injection time was determined by monoexponential extrapolation. The mean (SD) difference (MD) was -23 ml (183) or -0.6% (5.7%). Linear regression revealed PV2 = 0.92.PV1 + 226 (r = 0.92). The PV values corresponded well with data from other studies. In 26 surgical patients PV was determined using two methods: 1) the same as in healthy subjects and 2) using a modification of this method in whole blood (PVB). For PVB measurement blood was drawn through a cuvette-densitometer from an arterial line. Calculations were the same as in PV determination except for the use of hematocrit to achieve plasma concentrations of ICG from whole blood. In patients MD were -53 ml (144) or -1.3% (4.3) for PV and -19 ml (161) or -0.3% (5.1) for PVB. Comparing PVB and PV revealed MD = -113 ml (149) or -3.3% (4.2). The whole blood method is easier to perform and reduces blood waste to almost zero. In conclusion, ICG is a suitable tracer for PV determination.
我们研究了使用吲哚菁绿（ICG）测定人体血浆容量（PV）的可行性。在23名健康受试者中进行了重复的PV测量以测试重复性。将ICG（0.25mg/kg）静脉注射到一侧手臂，从另一侧手臂抽取静脉血。在密度计中测量第3至9分钟血浆样本的光密度。通过单指数外推法确定注射时的ICG浓度。平均（标准差）差值（MD）为-23ml（183）或-0.6%（5.7%）。线性回归显示PV2 = 0.92·PV1 + 226（r = 0.92）。PV值与其他研究的数据吻合良好。在26名外科手术患者中，使用两种方法测定PV：1）与健康受试者相同的方法；2）对全血采用该方法的改良版（PVB）。对于PVB测量，通过比色皿 - 密度计从动脉导管抽取血液。计算方法与PV测定相同，只是使用血细胞比容从全血中得出ICG的血浆浓度。在患者中，PV的MD为-53ml（144）或-1.3%（4.3），PVB的MD为-19ml（161）或-0.3%（5.1）。比较PVB和PV显示MD = -113ml（149）或-3.3%（4.2）。全血法操作更简便，且几乎将血液浪费降至零。总之，ICG是一种适用于PV测定的示踪剂。
