Feizi T, Lecomte J, Childs R, Solomon A
Scand J Immunol. 1976;5(6-7):629-36. doi: 10.1111/j.1365-3083.1976.tb03012.x.
In a search for H- or L-chain-related cross-idiotypic specificity among human anti-I and anti-i cold agglutinins, two idiotypic antisera raised against the IgMkappa cold agglutinin Da were tested for their binding activity to isolated cold agglutinin H and L chains. Negligible H-chain binding activity was found, but there was high-titre L-chain binding activity in one of the antisera. This was an unsuspected VkappaIII subgroup activity which enabled the classification of VkappaIII proteins into three subgroups. The kappa chains of five out of six anti-I and anti-i cold agglutinins belonged to the antigenically most active VkappaIII subgroup. Absorption of the idiotypic antiserum with a Bence Jones protein of this latter subgroup did not appreciably alter the precipitating cross-idiotypic activity of the antiserum when tested with intect cold agglutinins. However, these studies do not rule out the possible existence of a VkappaIII subgroup-associated conformational antigen in an intact Fab region, which is seen as a 'cross idiotypic' antigen by heterologous (rabbit anti-human) antisera.
为了在人类抗I和抗i冷凝集素中寻找与重链或轻链相关的交叉独特型特异性，针对IgMκ冷凝集素Da制备的两种独特型抗血清被检测其与分离出的冷凝集素重链和轻链的结合活性。发现重链结合活性可忽略不计，但其中一种抗血清存在高滴度的轻链结合活性。这是一种未被怀疑的VκIII亚群活性，它能够将VκIII蛋白分为三个亚群。六份抗I和抗i冷凝集素中的五份的κ链属于抗原性最活跃的VκIII亚群。用该后一个亚群的本-周蛋白吸收独特型抗血清后，当用完整的冷凝集素检测时，抗血清的沉淀交叉独特型活性没有明显改变。然而，这些研究并不排除在完整的Fab区域可能存在与VκIII亚群相关的构象抗原，异源（兔抗人）抗血清将其视为“交叉独特型”抗原。
