评估不同患者粪便标本混合的可靠性以及通过微量滴定酶联免疫吸附测定法检测蓝氏贾第鞭毛虫抗原。
Evaluation of reliability of pooling stool specimens from different patients and detection of Giardia lamblia antigen by microtiter enzyme-linked immunosorbent assay.
作者信息
McLaughlin J C, Rasmussen S K, Nims L J, Madar D A, Yazzie C R
机构信息
Department of Pathology, University of New Mexico School of Medicine, Albuquerque 87131.
出版信息
J Clin Microbiol. 1993 Oct;31(10):2807-8. doi: 10.1128/jcm.31.10.2807-2808.1993.
Abstract
We have shown that stool samples from different patients can be pooled at a 1:2 dilution and reliably assayed for Giardia lamblia antigen by a commercial microtiter enzyme-linked immunosorbent assay (ELISA) system (LMD Laboratories, Inc., Carlsbad, Calif.). Laboratories can reduce reagent costs by pooling specimens submitted for the detection of Giardia antigen by ELISA.
摘要
我们已经证明，来自不同患者的粪便样本可以按1:2的稀释度混合，并通过商业微量滴定酶联免疫吸附测定（ELISA）系统（LMD Laboratories, Inc., Carlsbad, Calif.）可靠地检测贾第鞭毛虫抗原。实验室可以通过混合提交用于ELISA检测贾第鞭毛虫抗原的标本，来降低试剂成本。
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