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Utility of a respiratory virus panel containing a monoclonal antibody pool for screening of respiratory specimens in nonpeak respiratory syncytial virus season.在非呼吸道合胞病毒流行季节,使用包含单克隆抗体库的呼吸道病毒检测板对呼吸道标本进行筛查的效用。
J Clin Microbiol. 1993 Oct;31(10):2809-11. doi: 10.1128/jcm.31.10.2809-2811.1993.
2
Reduction of nonspecific fluorescence in respiratory specimens by pretreatment with N-acetylcysteine.通过用N-乙酰半胱氨酸预处理降低呼吸道标本中的非特异性荧光。
J Clin Microbiol. 1986 Sep;24(3):470-1. doi: 10.1128/jcm.24.3.470-471.1986.
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[Detection of respiratory syncytial and adenovirus in nasopharyngeal aspirates: comparison of cellular cultures and immunofluorescence].[鼻咽抽吸物中呼吸道合胞病毒和腺病毒的检测:细胞培养与免疫荧光法的比较]
Rev Med Chil. 1992 Apr;120(4):415-9.
4
Evaluation of the Prodesse Hexaplex multiplex PCR assay for direct detection of seven respiratory viruses in clinical specimens.评估Prodesse六重多重PCR检测法用于直接检测临床标本中的七种呼吸道病毒。
Am J Clin Pathol. 2001 Aug;116(2):218-24. doi: 10.1309/F1R7-XD6T-RN09-1U6L.
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Evaluation of WHO monoclonal antibody kit for diagnosis of acute respiratory viral infections.世界卫生组织用于诊断急性呼吸道病毒感染的单克隆抗体试剂盒评估
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Sensitivity of respiratory virus culture when screening with R-mix fresh cells.使用R-mix新鲜细胞进行筛查时呼吸道病毒培养的敏感性。
J Clin Microbiol. 2004 Jan;42(1):79-82. doi: 10.1128/JCM.42.1.79-82.2004.
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Comparison of three techniques for detection of respiratory viruses in nasopharyngeal aspirates from children with lower acute respiratory infections.三种检测急性下呼吸道感染患儿鼻咽抽吸物中呼吸道病毒技术的比较。
J Med Virol. 1989 Jul;28(3):159-62. doi: 10.1002/jmv.1890280310.
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Evaluation of a monoclonal antibody pool for rapid diagnosis of respiratory viral infections.用于快速诊断呼吸道病毒感染的单克隆抗体组合的评估
J Clin Microbiol. 1989 Mar;27(3):448-52. doi: 10.1128/jcm.27.3.448-452.1989.
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Comparison of monoclonal antibody time-resolved fluoroimmunoassay with monoclonal antibody capture-biotinylated detector enzyme immunoassay for respiratory syncytial virus and parainfluenza virus antigen detection.单克隆抗体时间分辨荧光免疫测定法与单克隆抗体捕获-生物素化检测酶免疫测定法用于呼吸道合胞病毒和副流感病毒抗原检测的比较。
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Virologically confirmed population-based burden of hospitalization caused by respiratory syncytial virus, adenovirus, and parainfluenza viruses in children in Hong Kong.香港儿童呼吸道合胞病毒、腺病毒和副流感病毒所致基于人群的住院负担的病毒学确认。
Pediatr Infect Dis J. 2010 Dec;29(12):1088-92. doi: 10.1097/INF.0b013e3181e9de24.

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Efficacy and safety of interferon on neonates with respiratory syncytial virus pneumonia.干扰素对呼吸道合胞病毒肺炎新生儿的疗效及安全性
Exp Ther Med. 2020 Dec;20(6):220. doi: 10.3892/etm.2020.9350. Epub 2020 Oct 15.

本文引用的文献

1
Rapid detection of influenza virus by shell vial assay with monoclonal antibodies.利用单克隆抗体通过空斑试验快速检测流感病毒。
J Clin Microbiol. 1986 Oct;24(4):677-9. doi: 10.1128/jcm.24.4.677-679.1986.
2
Efficiency of immunofluorescence for rapid detection of common respiratory viruses.免疫荧光法快速检测常见呼吸道病毒的效率
J Clin Microbiol. 1987 Feb;25(2):355-7. doi: 10.1128/jcm.25.2.355-357.1987.
3
Routine viral culture for pediatric respiratory specimens submitted for direct immunofluorescence testing.提交用于直接免疫荧光检测的儿科呼吸道标本的常规病毒培养。
J Clin Microbiol. 1989 Jul;27(7):1438-40. doi: 10.1128/jcm.27.7.1438-1440.1989.
4
Evaluation of a monoclonal antibody pool for rapid diagnosis of respiratory viral infections.用于快速诊断呼吸道病毒感染的单克隆抗体组合的评估
J Clin Microbiol. 1989 Mar;27(3):448-52. doi: 10.1128/jcm.27.3.448-452.1989.
5
Evaluation of direct immunofluorescence, enzyme immunoassay, centrifugation culture, and conventional culture for the detection of respiratory syncytial virus.评估直接免疫荧光法、酶免疫测定法、离心培养法和传统培养法用于检测呼吸道合胞病毒的效果。
J Clin Microbiol. 1990 Nov;28(11):2394-7. doi: 10.1128/jcm.28.11.2394-2397.1990.
6
Comparison of enzyme-linked immunosorbent assay, indirect immunofluorescence assay, and virus isolation for detection of respiratory viruses in nasopharyngeal secretions.酶联免疫吸附测定、间接免疫荧光测定和病毒分离法检测鼻咽分泌物中呼吸道病毒的比较。
J Clin Microbiol. 1991 Mar;29(3):470-4. doi: 10.1128/jcm.29.3.470-474.1991.
7
Comparison of rapid immunofluorescence assay to cell culture isolation for the detection of influenza A and B viruses in nasopharyngeal secretions from infants and children.用于检测婴幼儿鼻咽分泌物中甲型和乙型流感病毒的快速免疫荧光测定法与细胞培养分离法的比较
J Virol Methods. 1991 Aug;33(3):305-10. doi: 10.1016/0166-0934(91)90030-4.
8
Rapid diagnosis of respiratory viral infections by using a shell vial assay and monoclonal antibody pool.使用空斑试验和单克隆抗体库快速诊断呼吸道病毒感染
J Clin Microbiol. 1992 Jun;30(6):1505-8. doi: 10.1128/jcm.30.6.1505-1508.1992.
9
Rapid detection of respiratory viruses by shell vial culture and direct staining by using pooled and individual monoclonal antibodies.通过空斑试验培养并使用混合及单克隆抗体进行直接染色来快速检测呼吸道病毒。
J Clin Microbiol. 1992 Mar;30(3):540-4. doi: 10.1128/jcm.30.3.540-544.1992.

在非呼吸道合胞病毒流行季节,使用包含单克隆抗体库的呼吸道病毒检测板对呼吸道标本进行筛查的效用。

Utility of a respiratory virus panel containing a monoclonal antibody pool for screening of respiratory specimens in nonpeak respiratory syncytial virus season.

作者信息

McDonald J C, Quennec P

机构信息

Department of Microbiology, Montreal Children's Hospital, McGill University, Quebec, Canada.

出版信息

J Clin Microbiol. 1993 Oct;31(10):2809-11. doi: 10.1128/jcm.31.10.2809-2811.1993.

DOI:10.1128/jcm.31.10.2809-2811.1993
PMID:8253993
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC266024/
Abstract

An indirect immunofluorescence respiratory virus panel containing monoclonal antibodies directed against respiratory syncytial virus, parainfluenza virus types 1, 2, and 3, adenovirus, and influenza viruses A and B was used to screen specimens in the nonpeak respiratory syncytial virus seasons in 1989 and 1990. The results indicate that the respiratory virus panel is fairly sensitive (79%) and very specific (99%) for the detection of respiratory viruses directly in clinical specimens during these time periods.

摘要

1989年和1990年呼吸道合胞病毒非流行季节期间,使用一种间接免疫荧光呼吸道病毒检测组合,其中包含针对呼吸道合胞病毒、1型、2型和3型副流感病毒、腺病毒以及甲型和乙型流感病毒的单克隆抗体,用于筛查标本。结果表明,在这些时间段内,该呼吸道病毒检测组合对于直接在临床标本中检测呼吸道病毒相当敏感(79%)且非常特异(99%)。