Jones S C, Evans S W, Lobo A J, Ceska M, Axon A T, Whicher J T
Centre for Digestive Diseases, General Infirmary, Leeds, United Kingdom.
J Gastroenterol Hepatol. 1993 Nov-Dec;8(6):508-12. doi: 10.1111/j.1440-1746.1993.tb01643.x.
To investigate the relationship between serum concentrations of interleukin-8 (IL-8) and disease activity in inflammatory bowel disease, serum IL-8 concentrations were measured by enzyme-linked immunosorbent assay (ELISA) in 93 patients. Interleukin-8 levels were compared with plasma interleukin-6 (IL-6) levels in 80 of these patients. Interleukin-8 levels were also measured in ten patients with active Crohn's disease, before and after treatment with a defined formula polymeric diet. Of these patients, 70 out of 93 IL-8 concentrations were below the detection limit of the assay. Levels were higher in patients with active ulcerative colitis (median < 20 pg/mL, 75th centile value = 190) compared with inactive disease (median and 75th centile value < 20; P < 0.05). Interleukin-8 concentrations correlated with a combined score for disease severity and extent (P = 0.01). Thirty-eight per cent (8/20) of patients with active Crohn's disease also had high levels of IL-8 but there was no significant difference between active and inactive disease. There was no correlation between serum IL-8 and plasma IL-6; on the contrary, very few patients had raised blood levels of both cytokines. In the diet treated group, serum IL-8 fell significantly after treatment (median = 37 pg/mL, range < 20-4615 before treatment, median < 20, range < 20-104 after treatment; P = 0.03). The results suggest that although IL-8 may be involved in the inflammatory process in inflammatory bowel disease, it is a poor marker of disease activity.
为了研究炎症性肠病患者血清白细胞介素-8（IL-8）浓度与疾病活动度之间的关系，采用酶联免疫吸附测定法（ELISA）检测了93例患者的血清IL-8浓度。其中80例患者的白细胞介素-8水平与血浆白细胞介素-6（IL-6）水平进行了比较。还对10例活动性克罗恩病患者在使用特定配方的聚合饮食治疗前后的白细胞介素-8水平进行了检测。在这93例患者中，有70例的IL-8浓度低于检测限。与非活动性疾病患者相比，活动性溃疡性结肠炎患者的IL-8水平更高（中位数<20 pg/mL，第75百分位数=190）（中位数和第75百分位数<20；P<0.05）。白细胞介素-8浓度与疾病严重程度和范围的综合评分相关（P=0.01）。38%（8/20）的活动性克罗恩病患者也有高水平的IL-8，但活动性疾病和非活动性疾病之间没有显著差异。血清IL-８与血浆IL-６之间没有相关性；相反，很少有患者两种细胞因子的血液水平都升高。在饮食治疗组中，治疗后血清IL-８显著下降（中位数=37 pg/mL，治疗前范围<20-4615，治疗后中位数<20，范围<20-104；P=0.03）。结果表明，虽然IL-８可能参与了炎症性肠病的炎症过程，但它不是疾病活动度的良好标志物。
