van Ketel R J, ter Schegget J, Zanen H C
J Clin Microbiol. 1984 Sep;20(3):362-4. doi: 10.1128/jcm.20.3.362-364.1984.
The DNA of patient and environmental isolates of Legionella pneumophila serogroup 1 was analyzed by restriction endonuclease cleavage. The electrophoretic patterns of the DNA digests of isolates from a group of patients with Legionnaires disease acquired in a hospital were indistinguishable from one another and were identical to the DNA pattern of a strain isolated from the hot water supply of the hospital. On the other hand, they were easily differentiated from strains isolated from patients and hot water supplies in other hospitals in the same city. The homogeneity of populations of L. pneumophila serogroup 1 colonizing plumbing systems was also investigated by DNA restriction endonuclease analysis in three hospitals. We distinguished two subtypes in one hospital; the two other hospitals had homogeneous populations. Restriction endonuclease digest analysis of L. pneumophila serogroup 1 DNA enables subtyping and appears to be a useful method for examining the epidemiology of outbreaks of Legionnaires disease.
通过限制性内切酶切割分析嗜肺军团菌血清1型患者分离株和环境分离株的DNA。从一家医院获得的一组军团病患者分离株的DNA消化物的电泳图谱彼此无法区分，并且与从该医院热水供应中分离出的菌株的DNA图谱相同。另一方面，它们很容易与从同一城市其他医院的患者和热水供应中分离出的菌株区分开来。还通过DNA限制性内切酶分析在三家医院研究了定殖于管道系统的嗜肺军团菌血清1型菌群的同质性。我们在一家医院区分出两个亚型；另外两家医院的菌群是同质的。嗜肺军团菌血清1型DNA的限制性内切酶消化分析能够进行亚型分析，似乎是检查军团病暴发流行病学的一种有用方法。
