Kalra R, Wade K E, Hands L, Styles P, Camplejohn R, Greenall M, Adams G E, Harris A L, Radda G K
MRC Radiobiology Unit, Chilton, Oxon, UK.
Br J Cancer. 1993 May;67(5):1145-53. doi: 10.1038/bjc.1993.211.
Phospholipid metabolism of human breast cancer was studied by 31P magnetic resonance spectroscopy (MRS). In vivo localised 31P MR spectra were obtained from the tumour alone using phase modulated rotating frame imaging. For 31 tumours, median (range) phosphomonoester (PME) to ATP ratio was 1.48 (0.57-3.78) and phosphodiester (PDE) to ATP ratio was 1.65 (0.44-3.89). DNA index and S phase fraction (SPF) were measured by flow cytometry of paraffin embedded tissue. Twelve (39%) tumours were diploid and 19 aneuploid. Median (range) SPF for 29 assessable tumours was 5.3% (0.6-28%), with significantly greater median SPF for aneuploid tumours (9.3%) than diploid (3.8%, P = 0.007). There was a significant association between PME/ATP and SPF (P = 0.03) due to a significant correlation for aneuploid tumours (P = 0.01). High resolution 31P MRS of extracts from 18 tumours (including seven studied in vivo) demonstrated that the PME peak consists predominantly of phosphoethanolamine (PE) with a smaller contribution from phosphocholine (PC) (median (range) PE/PC: 3.02 (1.13-5.09)). Changes in PME/ATP were observed for two tumours where tamoxifen stablized disease and may be consistent with the cytostatic effects of this drug.
采用³¹P磁共振波谱（MRS）研究了人类乳腺癌的磷脂代谢。使用相位调制旋转框架成像，仅从肿瘤中获取体内局部³¹P MR谱。对于31个肿瘤，磷酸单酯（PME）与ATP的中位数（范围）比值为1.48（0.57 - 3.78），磷酸二酯（PDE）与ATP的比值为1.65（0.44 - 3.89）。通过石蜡包埋组织的流式细胞术测量DNA指数和S期分数（SPF）。12个（39%）肿瘤为二倍体，19个为非整倍体。29个可评估肿瘤的SPF中位数（范围）为5.3%（0.6 - 28%），非整倍体肿瘤的SPF中位数（9.3%）显著高于二倍体肿瘤（3.8%，P = 0.007）。由于非整倍体肿瘤之间存在显著相关性（P = 0.01），PME/ATP与SPF之间存在显著关联（P = 0.03）。对18个肿瘤提取物进行的高分辨率³¹P MRS（包括7个体内研究肿瘤）表明，PME峰主要由磷酸乙醇胺（PE）组成，磷酸胆碱（PC）的贡献较小（中位数（范围）PE/PC：3.02（1.13 - 5.09））。在两个他莫昔芬使疾病稳定的肿瘤中观察到PME/ATP的变化，这可能与该药物的细胞生长抑制作用一致。
