Linderholm M, Ahlm C, Settergren B, Waage A, Tärnvik A
Department of Infectious Diseases, Umeå University, Sweden.
J Infect Dis. 1996 Jan;173(1):38-43. doi: 10.1093/infdis/173.1.38.
Plasma levels of cytokines were measured by EIA in 15 subjects hospitalized with nephropathia epidemica, a European form of hantavirus-induced hemorrhagic fever with renal syndrome. Concentrations of tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 were increased in all patients at admission, and the concentration of IL-10 was increased in most. TNF-alpha concentrations were still increased 1 week after onset of disease; levels of IL-6 and IL-10 were normalized. TNF-alpha was undetectable by the WEHI cell assay in serum samples obtained throughout the acute phase of disease. Serum levels of the two soluble TNF receptors p55 and p75 correlated with levels of the cytokine, indicating that receptor binding may be the reason for lack of bioactivity in vitro. TNF-alpha is known to induce pathophysiologic and clinical changes similar to those seen in nephropathia epidemica and in diseases caused by other hantaviruses.
采用酶免疫分析法（EIA）检测了15例因流行性肾病住院患者的血浆细胞因子水平。流行性肾病是汉坦病毒引起的肾综合征出血热的一种欧洲类型。所有患者入院时肿瘤坏死因子（TNF）-α和白细胞介素（IL）-6浓度均升高，大多数患者IL-10浓度升高。疾病发作1周后TNF-α浓度仍升高；IL-6和IL-10水平恢复正常。在疾病急性期采集的血清样本中，通过WEHI细胞试验未检测到TNF-α。两种可溶性TNF受体p55和p75的血清水平与细胞因子水平相关，表明受体结合可能是体外缺乏生物活性的原因。已知TNF-α可诱导与流行性肾病及其他汉坦病毒引起的疾病中所见相似的病理生理和临床变化。
