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Comparison of radioimmunoassay and the gel filtration technique for routine diagnosis of rubella during pregnancy.放射免疫测定法与凝胶过滤技术在孕期风疹常规诊断中的比较。
J Hyg (Lond). 1981 Oct;87(2):249-55. doi: 10.1017/s0022172400069461.
2
The detection of rubella-specific IgM antibodies by radioimmunoassay.采用放射免疫测定法检测风疹特异性IgM抗体。
Br J Exp Pathol. 1978 Dec;59(6):577-83.
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[Studies on the management of pregnant women having high hemagglutination inhibition (HI) antibody titer of rubella virus in the first trimester examination of rubella virus antibody in the pregnant women having high HI titer by different methods and prognosis of their infants after birth].[关于妊娠早期风疹病毒血凝抑制（HI）抗体效价高的孕妇风疹病毒抗体检测不同方法的管理及出生后婴儿预后的研究]
Nihon Sanka Fujinka Gakkai Zasshi. 1987 Nov;39(11):1993-9.
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Evaluation of a new ELISA method for estimating rubella-specific IgM antibody.一种用于评估风疹特异性IgM抗体的新型酶联免疫吸附测定（ELISA）方法
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The detection of specific IgM antibodies following infection with rubella virus.风疹病毒感染后特异性IgM抗体的检测。
J Clin Pathol. 1975 May;28(5):377-82. doi: 10.1136/jcp.28.5.377.
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Completion of hemagglutination inhibition test by solid-phase radioimmunoassay test in routine diagnostic rubella serology.通过固相放射免疫测定法完成常规诊断风疹血清学中的血凝抑制试验。
Med Biol. 1977 Aug;55(4):241-4.
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Detection of specific IgG and IgM antibodies in the haemagglutination inhibition test and the enzyme-linked immunoassay for the diagnosis of rubella infection.在血凝抑制试验和酶联免疫吸附测定中检测特异性IgG和IgM抗体以诊断风疹感染。
Infection. 1986 Jul-Aug;14(4):159-62. doi: 10.1007/BF01645254.
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How to diagnose rubella during pregnancy.如何在孕期诊断风疹。
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Evaluation of a simplified sucrose gradient method for the detection of rubella-specific IgM in routine diagnostic practice.一种简化蔗糖梯度法在常规诊断实践中检测风疹特异性 IgM 的评估。
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本文引用的文献

1
Rubella screening tests.风疹筛查试验
J Clin Pathol. 1973 Feb;26(2):161-2. doi: 10.1136/jcp.26.2.161.
2
A simplified method for the detection of rubella-specific IgM employing sucrose density fractionation and 2-mercaptoethanol.一种采用蔗糖密度分级分离和2-巯基乙醇检测风疹特异性IgM的简化方法。
J Hyg (Lond). 1974 Dec;73(3):329-40. doi: 10.1017/s0022172400042674.
3
Serum IgM and IgG responses in postnatally acquired rubella.出生后获得性风疹的血清IgM和IgG反应。
Lancet. 1969 Jul 12;2(7611):65-8. doi: 10.1016/s0140-6736(69)92386-1.
4
The detection of specific IgM antibodies following infection with rubella virus.风疹病毒感染后特异性IgM抗体的检测。
J Clin Pathol. 1975 May;28(5):377-82. doi: 10.1136/jcp.28.5.377.
5
The routine serological investigation of cases and contacts of rubella.风疹病例及接触者的常规血清学调查。
J Hyg (Lond). 1975 Aug;75(1):91-8. doi: 10.1017/s0022172400047100.
6
The detection and avoidance of false-positive reactions in tests for rubella-specific IgM.风疹特异性 IgM 检测中假阳性反应的检测与避免
J Med Microbiol. 1976 Aug;9(3):355-7. doi: 10.1099/00222615-9-3-355.
7
Solid-phase radioimmunoassay of rubella virus immunoglobulin M antibodies: comparison with sucrose density gradient centrifugation test.风疹病毒免疫球蛋白M抗体的固相放射免疫测定：与蔗糖密度梯度离心试验的比较
J Clin Microbiol. 1977 Mar;5(3):257-62. doi: 10.1128/jcm.5.3.257-262.1977.
8
The detection of rubella-specific IgM antibodies by radioimmunoassay.采用放射免疫测定法检测风疹特异性IgM抗体。
Br J Exp Pathol. 1978 Dec;59(6):577-83.
9
Comparison of methods for detecting specific IgM antibody in infants with congenital rubella.先天性风疹婴儿中特异性IgM抗体检测方法的比较
J Med Microbiol. 1978 Nov;11(4):411-8. doi: 10.1099/00222615-11-4-411.
10
Comparison of immunofluorescence and radioimmunoassay for detecting IgM antibody in infants with the congenital rubella syndrome.免疫荧光法与放射免疫分析法在检测先天性风疹综合征婴儿IgM抗体中的比较。
J Hyg (Lond). 1979 Dec;83(3):413-23. doi: 10.1017/s0022172400026243.

放射免疫测定法与凝胶过滤技术在孕期风疹常规诊断中的比较。

Comparison of radioimmunoassay and the gel filtration technique for routine diagnosis of rubella during pregnancy.

作者信息

Kangro H O, Jackson C, Heath R B

出版信息

J Hyg (Lond). 1981 Oct;87(2):249-55. doi: 10.1017/s0022172400069461.

DOI:10.1017/s0022172400069461
PMID:7288178
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2134043/
Abstract

Radioimmunoassay (RIA) for rubella-specific IgM antibodies was compared with haemagglutination-inhibition (HI) in conjunction with gel filtration for the diagnosis of rubella infection in pregnant women during a 1-year period. In total 476 women were investigated of whom 221 were tested for rubella IgM. Both techniques gave positive results with 64 sera, and RIA alone with one additional serum. Difficulties associated with the removal of non-specific HI activity were encountered with four sera all of which were negative by RIA. RIA was found to have practical advantages over the gel filtration method but is at present technically more difficult to perform.

摘要

在为期1年的时间里，对放射免疫分析法（RIA）检测风疹特异性IgM抗体与血凝抑制试验（HI）联合凝胶过滤法用于诊断孕妇风疹感染的情况进行了比较。总共对476名女性进行了调查，其中221名检测了风疹IgM。两种技术对64份血清检测结果均为阳性，仅RIA法对另外1份血清检测为阳性。4份血清在去除非特异性HI活性方面存在困难，RIA法检测这4份血清均为阴性。结果发现，RIA法比凝胶过滤法具有实际优势，但目前在技术操作上更困难。