Osawa R, Okitsu T, Morozumi H, Yamai S
Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratories, Japan.
Appl Environ Microbiol. 1996 Feb;62(2):725-7. doi: 10.1128/aem.62.2.725-727.1996.
A total of 132 strains of V. parahaemolyticus isolated from patients and from the suspected causal food items of past food poisoning cases occurring in Kanagawa Prefecture, Japan, were examined for the ability to hydrolyze urea, with specific reference to the presence of the thermostable direct hemolysin gene (tdh) and the gene for thermostable direct hemolysin-related hemolysin (trh). Ten strains belonging to five different O-antigen serotypes were positive for urea hydrolysis (UH+), and four of these strains did not carry tdh. A total of 106 strains carried tdh, but less than 6% of them were UH+, whereas all trh-carrying strains were UH+. The evidence suggests that urea hydrolysis is not a reliable marker for identifying tdh-carrying V. parahaemolyticus strains in Japan (the Pacific Northeast) but may be a marker for trh-carrying strains.
对从日本神奈川县过去食物中毒病例的患者及疑似致病食品中分离出的132株副溶血性弧菌进行了尿素水解能力检测,并特别检测了耐热直接溶血素基因(tdh)和耐热直接溶血素相关溶血素基因(trh)的存在情况。属于五种不同O抗原血清型的10株菌尿素水解呈阳性(UH+),其中4株不携带tdh。共有106株携带tdh,但其中UH+的菌株不到6%,而所有携带trh的菌株均为UH+。有证据表明,在日本(太平洋东北部),尿素水解并非鉴定携带tdh的副溶血性弧菌菌株的可靠标志物,但可能是携带trh菌株的标志物。