Twombly V, Blackman R K, Jin H, Graff J M, Padgett R W, Gelbart W M
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
Development. 1996 May;122(5):1555-65. doi: 10.1242/dev.122.5.1555.
We examine roles of signaling by secreted ligands of the TGF-beta family during Drosophila oogenesis. One family member, the DPP ligand encoded by the decapentaplegic (dpp) gene, is required for patterning of anterior eggshell structures. This requirement presumably reflects the expression pattern of dpp in an anterior subset of somatic follicle cells: the centripetally migrating and the nurse cell-associated follicle cells. Similar requirements are also revealed by mutations in the saxophone (sax)-encoded receptor, consistent with the idea that DPP signaling is, at least in part, mediated by the SAX receptor. A loss of germline sax function results in a block in oogenesis associated with egg chamber degeneration and a failure of the transfer of nurse cell contents to the oocyte, indicating that TGF-beta signaling is required for these events. Some phenotypes of sax mutations during oogenesis suggest that SAX responds to at least one other TGF-beta ligand as well in the posterior follicle cells.
我们研究了转化生长因子-β（TGF-β）家族分泌配体的信号传导在果蝇卵子发生过程中的作用。该家族的一个成员，由“脱缰蛋白”（dpp）基因编码的DPP配体，是前侧卵壳结构模式形成所必需的。这种需求可能反映了dpp在体细胞滤泡细胞前侧亚群中的表达模式：向心迁移的细胞和与滋养细胞相关的滤泡细胞。“萨克斯管”（sax）编码的受体发生突变也显示出类似的需求，这与DPP信号传导至少部分由SAX受体介导的观点一致。生殖系sax功能丧失会导致卵子发生受阻，伴有卵室退化，以及滋养细胞内容物向卵母细胞的转移失败，这表明TGF-β信号传导是这些事件所必需的。卵子发生过程中sax突变的一些表型表明，SAX在后侧滤泡细胞中也对至少一种其他TGF-β配体作出反应。
