Effect of vitamin E and selenium on hypothermic restraint stress and chemically-induced ulcers.

The present study was undertaken to determine the effect of a combination of selenium and vitamin E on stress and chemical-induced gastric ulcers in rats. The gastric mucosal lesions were produced by hypothermic restraint stress, indomethacin, reserpine, and mucosal damaging agents including 80% ethanol, 0.6 M HCl, 25% NaCl, and 0.2 M NaOH. The gastric secretion studies were undertaken using Shay's pylorus ligation model. The results of this study demonstrated that the treatment of rats with selenium or vitamin E significantly reduced the basal gastric acid secretions when given individually; however, the combination of these agents produced a better inhibition of gastric acid secretions as compared to their individual effect. Both selenium and vitamin E were found to protect gastric mucosa against the lesions produced by hypothermic restraint stress and chemicals, but a highly significant protection was observed when they were used concomitantly. Vitamin E alone and with selenium significantly inhibited ethanol-induced depletion of gastric nonprotein sulfhydryl compounds. Our findings also showed that the combination of selenium and vitamin E provided better protection to gastric mucosa against hypothermic restraint-induced gastric wall mucus depletion. This study clearly suggests the feasibility of using selenium and vitamin E concurrently to achieve better gastroprotective effects.