Suppr超能文献

中国仓鼠卵巢染色质的结构重复单元。高等真核生物中重复单元DNA大小变化的证据。

Structural repeat units of Chinese hamster ovary chromatin. Evidence for variations in repeat unit DNA size in higher eukaryotes.

作者信息

Rill R L, Nelson D A, Oosterhof D K, Hozier J C

出版信息

Nucleic Acids Res. 1977 Apr;4(4):771-89. doi: 10.1093/nar/4.4.771.

DOI:10.1093/nar/4.4.771
PMID:866190
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342483/
Abstract

DNA lengths in the structural repeat units of Chinese hamster ovary (CHO) and chicken erythrocyte chromatin were compared by analyzing the sizes of DNA fragments produced after treatment of nuclei with staphylococcal nuclease. The repeat length of CHO chromatin (173 +- 4 BP) is about 20 base pairs (BP) smaller than that of chicken erythrocyte chromatin (194 +- 8 BP). Repeat lengths of rat liver and calf thymus chromatin were found to be about 10 BP shorter than that of chicken erythrocyte chromatin. Thus significant variations occur in repeat units of chromatin of higher eukaryotes. These variations occur in the lengths of "spacer" (or "internucleosomal") DNA segments, not in "core particle" (or "nucleosomal") DNA lengths. The concept of spacer regions and the possible influence of H1 histones is discussed.

摘要

通过分析用葡萄球菌核酸酶处理细胞核后产生的DNA片段大小，比较了中国仓鼠卵巢（CHO）和鸡红细胞染色质结构重复单元中的DNA长度。CHO染色质的重复长度（173±4碱基对）比鸡红细胞染色质的重复长度（194±8碱基对）小约20个碱基对（BP）。发现大鼠肝脏和小牛胸腺染色质的重复长度比鸡红细胞染色质的重复长度短约10 BP。因此，高等真核生物染色质的重复单元存在显著差异。这些差异发生在“间隔”（或“核小体间”）DNA片段的长度上，而不是“核心颗粒”（或“核小体”）DNA的长度上。文中讨论了间隔区的概念以及H1组蛋白可能产生的影响。

相似文献

1
Structural repeat units of Chinese hamster ovary chromatin. Evidence for variations in repeat unit DNA size in higher eukaryotes.中国仓鼠卵巢染色质的结构重复单元。高等真核生物中重复单元DNA大小变化的证据。
Nucleic Acids Res. 1977 Apr;4(4):771-89. doi: 10.1093/nar/4.4.771.
2
DNA repeat lengths of erythrocyte chromatins differing in content of histones H1 and H5.组蛋白H1和H5含量不同的红细胞染色质的DNA重复长度
Nucleic Acids Res. 1980 Feb 11;8(3):529-42. doi: 10.1093/nar/8.3.529.
3
Protection of discrete DNA fragments by the complex H1-octamerhistones or H5-octamerhistones after micrococcal nuclease digestion.微球菌核酸酶消化后，由H1-八聚体组蛋白或H5-八聚体组蛋白复合物对离散DNA片段的保护作用。
Nucleic Acids Res. 1981 Aug 11;9(15):3671-80. doi: 10.1093/nar/9.15.3671.
4
Comparative subunit structure of HeLa, yeast, and chicken erythrocyte chromatin.海拉细胞、酵母和鸡红细胞染色质的比较亚基结构。
Proc Natl Acad Sci U S A. 1977 Jan;74(1):79-83. doi: 10.1073/pnas.74.1.79.
5
Structure of nucleosomes and organization of internucleosomal DNA in chromatin.染色质中核小体的结构及核小体间DNA的组织形式
J Mol Biol. 1990 Apr 5;212(3):495-511. doi: 10.1016/0022-2836(90)90328-J.
6
Biochemical evidence of variability in the DNA repeat length in the chromatin of higher eukaryotes.高等真核生物染色质中DNA重复长度变异性的生化证据。
Proc Natl Acad Sci U S A. 1976 Dec;73(12):4382-6. doi: 10.1073/pnas.73.12.4382.
7
The interaction of H1 histone with nucleosome core.H1组蛋白与核小体核心的相互作用。
J Biochem. 1981 Jun;89(6):1881-8. doi: 10.1093/oxfordjournals.jbchem.a133390.
8
The effects of salt concentration and H-1 depletion on the digestion of calf thymus chromatin by micrococcal nuclease.盐浓度和H-1缺失对微球菌核酸酶消化小牛胸腺染色质的影响。
Nucleic Acids Res. 1979;6(5):1843-62. doi: 10.1093/nar/6.5.1843.
9
A comparison of the structure of chicken erythrocyte and chicken liver chromatin.鸡红细胞与鸡肝染色质结构的比较。
Cell. 1976 Dec;9(4 Pt 1):627-32. doi: 10.1016/0092-8674(76)90045-3.
10
Micrococcal nuclease cleavage of chromatin displays nonrandom properties.微球菌核酸酶对染色质的切割表现出非随机特性。
Biochemistry. 1981 Apr 14;20(8):2127-32. doi: 10.1021/bi00511a009.

引用本文的文献

1
Organizational changes in chromatin at different malignant stages of Friend erythroleukemia.弗瑞德红白血病不同恶性阶段染色质的组织学变化
Nucleic Acids Res. 1980 Nov 25;8(22):5317-31. doi: 10.1093/nar/8.22.5317.
2
DNA repeat lengths of erythrocyte chromatins differing in content of histones H1 and H5.组蛋白H1和H5含量不同的红细胞染色质的DNA重复长度
Nucleic Acids Res. 1980 Feb 11;8(3):529-42. doi: 10.1093/nar/8.3.529.
3
Methylation of nucleosomal and nuclease sensitive DNA.核小体及核酸酶敏感DNA的甲基化
Nucleic Acids Res. 1977 Sep;4(9):3097-108. doi: 10.1093/nar/4.9.3097.
4
Histones H2A and H2B are neighbors along DNA in chromatin: characterization of subnucleosomal particles containing H2A+H2B.组蛋白H2A和H2B在染色质中沿DNA相邻：含H2A + H2B的亚核小体颗粒的特性
Nucleic Acids Res. 1977 Dec;4(12):4223-34. doi: 10.1093/nar/4.12.4223.

本文引用的文献

1
Preparation of native chromatin and damage caused by shearing.制备天然染色质和剪切引起的损伤。
Science. 1975 Mar 28;187(4182):1203-6. doi: 10.1126/science.187.4182.1203.
2
The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.用聚丙烯酰胺凝胶电泳法分离高分子量核糖核酸
Biochem J. 1967 Jan;102(1):251-7. doi: 10.1042/bj1020251.
3
The effect of trypsin on nuclease-resistant chromatin fragments.胰蛋白酶对核酸酶抗性染色质片段的作用。
J Biol Chem. 1974 Jan 10;249(1):152-6.
4
Chromatin structure: a repeating unit of histones and DNA.染色质结构：组蛋白与DNA的重复单元。
Science. 1974 May 24;184(4139):868-71. doi: 10.1126/science.184.4139.868.
5
Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease.染色质亚结构。一种核脱氧核糖核酸酶对染色质DNA在规则间隔位点的消化作用。
Biochem Biophys Res Commun. 1973 May 15;52(2):504-10. doi: 10.1016/0006-291x(73)90740-7.
6
Electron microscopy of chromatin subunit particles.染色质亚基颗粒的电子显微镜检查。
Biochem Biophys Res Commun. 1974 Oct 23;60(4):1365-70. doi: 10.1016/0006-291x(74)90348-9.
7
Mammalian chromatin substructure studies with the calcium-magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis.利用钙镁内切核酸酶和二维聚丙烯酰胺凝胶电泳进行的哺乳动物染色质亚结构研究。
Biochem J. 1974 Oct;143(1):67-72. doi: 10.1042/bj1430067.
8
Chromatographic separation of chromatin subunits.染色质亚基的色谱分离
Biochem Biophys Res Commun. 1974 Dec 23;61(4):1193-8. doi: 10.1016/s0006-291x(74)80410-9.
9
Subunit structure of chromatin.染色质的亚基结构。
Nature. 1974 Sep 20;251(5472):249-51. doi: 10.1038/251249a0.
10
Chemical probes of chromatin structure.染色质结构的化学探针
Biochemistry. 1974 Aug 13;13(17):3622-8. doi: 10.1021/bi00714a034.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验