Bleumink-Pluym N M, ter Laak E A, Houwers D J, van der Zeijst B A
Department of Bacteriology, The Netherlands.
Clin Diagn Lab Immunol. 1996 Jan;3(1):47-50. doi: 10.1128/cdli.3.1.47-50.1996.
The ability of Taylorella equigenitalis, the causative agent of contagious equine metritis, to invade and replicate in equine derm cells was studied. The kinetics of invasion and replication were determined for four T. equigenitalis strains. On the basis of these experiments, a simpler assay in which the invasive as well as the replicative properties of a particular strain could be determined was developed. This assay was used to characterize 32 strains, which had previously been typed by field inversion gel electrophoresis of genomic restriction fragments. The invasiveness of T. equigenitalis strains ranged from 3 to 0.015 bacteria per cell and seemed to be associated with the contagiousness of the infection. The replication index (number of intracellular bacteria per cell at 24 h after inoculation divided by the number of intracellular bacteria per cell at 4 h after inoculation) varied from 1 to 857 and seemed to be associated with the severity of the symptoms of contagious equine metritis. There was no association between the invasiveness and the replication index of the strains, nor was there an association of invasion and replication with field inversion gel electrophoresis grouping.
研究了马传染性子宫炎的病原体马泰勒氏菌侵入马皮肤细胞并在其中复制的能力。测定了四株马泰勒氏菌的侵入和复制动力学。基于这些实验，开发了一种更简单的检测方法，可用于确定特定菌株的侵入性和复制特性。该检测方法用于鉴定32株菌株，这些菌株此前已通过基因组限制性片段的场反转凝胶电泳进行分型。马泰勒氏菌菌株的侵入性范围为每个细胞3至0.015个细菌，似乎与感染的传染性有关。复制指数（接种后24小时每个细胞内细菌数除以接种后4小时每个细胞内细菌数）在1至857之间变化，似乎与马传染性子宫炎症状的严重程度有关。菌株的侵入性与复制指数之间没有关联，侵入和复制与场反转凝胶电泳分组也没有关联。
