Lozniewski A, De Korwin J D, Conroy M C, Plenat F, Weber M
Laboratoire de Bactériologie, Hôpital Central, Centre Hospitalier et Universitaire, Nancy, France.
J Clin Microbiol. 1996 Jul;34(7):1773-5. doi: 10.1128/JCM.34.7.1773-1775.1996.
We evaluated the performance of a new latex agglutination test, Pyloriset Dry (Orion Diagnostica, Espoo, Finland), in the simultaneous detection of immunoglobulin G (IgG), IgA, and IgM antibodies to Helicobacter pylori and compared it with that of the Pyloristat test (BioWhittaker, Fontenay-sous-Bois, France), an enzyme-linked immunosorbent assay detecting IgG to H. pylori, for 96 untreated dyspeptic patients who had undergone gastroduodenal endoscopy. Infection was diagnosed in 56 cases by positive culture and/or positive Giemsa stain and rapid urease test (antral biopsies) and was associated with chronic gastritis in 52 patients. Forty noninfected patients did not have chronic gastritis. The sensitivity of Pyloriset Dry was 91.1%. The sensitivity of Pyloristat was 91.1 or 82.1%, depending on whether equivocal results were considered positive or negative, respectively. Both tests had a specificity of 87.5%. Their performances were not statistically different. Thus, Pyloriset Dry is an alternative to serological tests for adults, particularly when a small number of serum samples has to be tested.
我们评估了一种新型乳胶凝集试验——Pyloriset Dry（芬兰埃斯波的奥立安诊断公司生产）在同时检测幽门螺杆菌免疫球蛋白G（IgG）、IgA和IgM抗体方面的性能，并将其与Pyloristat试验（法国丰特奈苏布瓦的生物惠特克公司生产）进行了比较。Pyloristat试验是一种检测幽门螺杆菌IgG的酶联免疫吸附测定法。我们对96例接受了胃十二指肠内镜检查的未经治疗的消化不良患者进行了检测。通过阳性培养和/或吉姆萨染色及快速尿素酶试验（取自胃窦的活检组织）确诊56例感染病例，其中52例患者伴有慢性胃炎。40例未感染患者无慢性胃炎。Pyloriset Dry的敏感性为91.1%。Pyloristat的敏感性分别为91.1%或82.1%，这取决于对可疑结果分别视为阳性或阴性。两种试验的特异性均为87.5%。它们的性能在统计学上无差异。因此，对于成人而言，Pyloriset Dry是血清学检测的一种替代方法，尤其是在需要检测少量血清样本时。
