Kornguth M L, Kunin C M
Antimicrob Agents Chemother. 1977 Jun;11(6):974-7. doi: 10.1128/AAC.11.6.974.
Rabbits were injected intramuscularly with gentamicin and amikacin (15 mg of base per kg), and the antibiotic levels in tissues were determined 20 h after either a single or multiple injections. Disk diffusion assays, with Bacillus subtilis ATCC 6633, were carried out both on homogenates and on supernatants deproteinized with trichloroacetic acid. Both assays indicated that the kidney is the major site of antibiotic deposition. Antibiotic levels increased after multiple doses. Gentamicin levels in other tissues were higher than those of amikacin. The assay of trichloroacetic acid-treated material was more sensitive than the assay of the total homogenate.
给兔子肌肉注射庆大霉素和阿米卡星（每千克体重15毫克碱基），单次或多次注射后20小时测定组织中的抗生素水平。使用枯草芽孢杆菌ATCC 6633进行纸片扩散试验，分别对匀浆和用三氯乙酸脱蛋白的上清液进行检测。两种检测均表明肾脏是抗生素沉积的主要部位。多次给药后抗生素水平升高。庆大霉素在其他组织中的水平高于阿米卡星。三氯乙酸处理材料的检测比总匀浆检测更灵敏。
