Rozenberg F, Lebon P
Laboratoire de recherche sur les infections virales, Hopital Saint-Vincent de Paul et Université René Descartes 82, Paris, France.
J Neurovirol. 1996 Aug;2(4):289-95. doi: 10.3109/13550289609146892.
Viral factors responsible for HSV neurovirulence in humans are still unknown. The aim of this work was to investigate the hypothesis that viral variants might contribute to the specific neurovirulence of some HSV strains. HSV-1 DNA was recovered from cerebrospinal fluid (CSF) in ten patients with HSV encephalitis (HSE) and the regions of HSV-1 gD gene corresponding to known antigenic sites were analyzed by direct sequencing of PCR products. Twenty-two mutations were found among a total of 6580 bp analyzed over a portion of 1000 bp of gD gene, of which 20 were silent whereas two conferred amino acid substitution. One missense mutation (E117D) was found in two CSF samples as well as in two control laboratory strains. The other one (A269T) was found in a single CSF sample, and lies within a region corresponding to a functionally essential antigenic site. These are the first mutations of the gene encoding gD of HSV identified in vivo in human encephalitis samples. Overall, the results argue against the role of gD in neurovirulence in humans.
导致人类单纯疱疹病毒（HSV）神经毒性的病毒因素仍不明确。本研究的目的是探讨病毒变异体可能导致某些HSV毒株具有特定神经毒性这一假说。从10例HSV脑炎（HSE）患者的脑脊液（CSF）中提取HSV-1 DNA，并通过对PCR产物进行直接测序，分析HSV-1 gD基因中与已知抗原位点相对应的区域。在gD基因1000 bp片段的总共6580 bp分析区域中发现了22个突变，其中20个是沉默突变，2个导致氨基酸替换。在两份脑脊液样本以及两份对照实验室毒株中发现了一个错义突变（E117D）。另一个（A269T）在单个脑脊液样本中发现，位于与功能必需抗原位点相对应的区域内。这些是在人类脑炎样本体内首次鉴定出的HSV gD编码基因的突变。总体而言，结果表明gD在人类神经毒性中不起作用。
