限制水溶性自旋标记物扩散的因素。
Factors restricting diffusion of water-soluble spin labels.
作者信息
Keith A D, Snipes W, Mehlhorn R J, Gunter T
出版信息
Biophys J. 1977 Sep;19(3):205-18. doi: 10.1016/S0006-3495(77)85582-3.
Abstract
Line broadening of spin label signals is treated in terms of concentration, viscosity, charge and temperature dependencies. Line broadening of spin label signals may be caused either by spin label interactions or by the interaction between a spin label and a second paramagnetic species. Line broadening has been related to collision frequency in the literature and is treated in that way here. Collision frequency is related to diffusion processes in a way that allows information to be obtained about the diffusion environment. Several potential spin label line-broadening agents are compared as to their effectiveness. Small polymer beads with graduated pore sizes are used to show that collisional broadening has a marked dependence on the long-range structure of the diffusion environment. Application of these results to biological diffusion processes is considered.
摘要
自旋标记信号的谱线展宽根据浓度、粘度、电荷和温度依赖性进行处理。自旋标记信号的谱线展宽可能是由自旋标记相互作用或自旋标记与第二种顺磁物质之间的相互作用引起的。在文献中，谱线展宽与碰撞频率有关，本文也采用这种方式进行处理。碰撞频率与扩散过程相关，通过这种方式可以获得有关扩散环境的信息。比较了几种潜在的自旋标记谱线展宽剂的有效性。使用具有不同孔径的小聚合物珠来表明碰撞展宽对扩散环境的长程结构有显著依赖性。考虑了这些结果在生物扩散过程中的应用。
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