限制水溶性自旋标记物扩散的因素。
Factors restricting diffusion of water-soluble spin labels.
作者信息
Keith A D, Snipes W, Mehlhorn R J, Gunter T
出版信息
Biophys J. 1977 Sep;19(3):205-18. doi: 10.1016/S0006-3495(77)85582-3.
Abstract
Line broadening of spin label signals is treated in terms of concentration, viscosity, charge and temperature dependencies. Line broadening of spin label signals may be caused either by spin label interactions or by the interaction between a spin label and a second paramagnetic species. Line broadening has been related to collision frequency in the literature and is treated in that way here. Collision frequency is related to diffusion processes in a way that allows information to be obtained about the diffusion environment. Several potential spin label line-broadening agents are compared as to their effectiveness. Small polymer beads with graduated pore sizes are used to show that collisional broadening has a marked dependence on the long-range structure of the diffusion environment. Application of these results to biological diffusion processes is considered.
摘要
自旋标记信号的谱线展宽根据浓度、粘度、电荷和温度依赖性进行处理。自旋标记信号的谱线展宽可能是由自旋标记相互作用或自旋标记与第二种顺磁物质之间的相互作用引起的。在文献中，谱线展宽与碰撞频率有关，本文也采用这种方式进行处理。碰撞频率与扩散过程相关，通过这种方式可以获得有关扩散环境的信息。比较了几种潜在的自旋标记谱线展宽剂的有效性。使用具有不同孔径的小聚合物珠来表明碰撞展宽对扩散环境的长程结构有显著依赖性。考虑了这些结果在生物扩散过程中的应用。
相似文献
1
Factors restricting diffusion of water-soluble spin labels.限制水溶性自旋标记物扩散的因素。
Biophys J. 1977 Sep;19(3):205-18. doi: 10.1016/S0006-3495(77)85582-3.
2
Linewidth analysis of spin labels in liquids. II. Experimental.
J Magn Reson. 1999 Jun;138(2):210-9. doi: 10.1006/jmre.1999.1738.
3
Diffusion of oxygen in water and hydrocarbons using an electron spin resonance spin-label technique.利用电子自旋共振自旋标记技术研究氧气在水和碳氢化合物中的扩散
Biophys J. 1984 Apr;45(4):743-8. doi: 10.1016/S0006-3495(84)84217-4.
4
[Study of the flexibility of serum albumin molecules using the spin label method].
Biofizika. 1985 Sep-Oct;30(5):772-6.
5
6
[Interaction of a membrane preparation of Na+, K+-ATPase with a spin-labeled analog of ATP].
Biokhimiia. 1977 Mar;42(3):476-80.
7
Diffusion of a small molecule in the cytoplasm of mammalian cells.小分子在哺乳动物细胞质中的扩散。
Proc Natl Acad Sci U S A. 1984 Jun;81(11):3414-8. doi: 10.1073/pnas.81.11.3414.
8
Saturation-recovery electron paramagnetic resonance discrimination by oxygen transport (DOT) method for characterizing membrane domains.用于表征膜结构域的基于氧传输（DOT）方法的饱和恢复电子顺磁共振鉴别
Methods Mol Biol. 2007;398:143-57. doi: 10.1007/978-1-59745-513-8_11.
9
Rotational diffusion of TEMPONE in the cytoplasm of Chinese hamster lung cells.TEMPONE在中国仓鼠肺细胞胞质中的旋转扩散。
Biophys J. 1983 Dec;44(3):405-12. doi: 10.1016/S0006-3495(83)84314-8.
10
A neutral water-soluble broadening agent for spin-label studies.
J Magn Reson B. 1994 May;104(1):69-72. doi: 10.1006/jmrb.1994.1054.
引用本文的文献
1
Electron paramagnetic resonance line shifts and line shape changes due to spin exchange of nitroxide free radicals in liquids. 7. Singly charged surfactant nitroxide.液体中氮氧自由基自旋交换导致的电子顺磁共振谱线位移和线形变化。7. 单电荷表面活性剂氮氧自由基
J Phys Chem A. 2009 Aug 20;113(33):9295-303. doi: 10.1021/jp905335r.
2
Localization of spin labels in oat leaf protoplasts.自旋标记在燕麦叶原生质体中的定位。
Plant Physiol. 1982 Sep;70(3):662-7. doi: 10.1104/pp.70.3.662.
3
Femtosecond dynamics of intracellular water probed with nonlinear optical Kerr effect microspectroscopy.利用非线性光学克尔效应显微光谱探测细胞内水的飞秒动力学。
Biophys J. 2001 Jun;80(6):3019-24. doi: 10.1016/S0006-3495(01)76267-4.
4
Determinants of the translational mobility of a small solute in cell cytoplasm.小溶质在细胞质中平移流动性的决定因素。
J Cell Biol. 1993 Jan;120(1):175-84. doi: 10.1083/jcb.120.1.175.
5
Lateral diffusion in an archipelago. Effects of impermeable patches on diffusion in a cell membrane.群岛中的横向扩散。不可渗透斑块对细胞膜扩散的影响。
Biophys J. 1982 Aug;39(2):165-73. doi: 10.1016/S0006-3495(82)84504-9.
6
Calcium translocation mechanism in sarcoplasmic reticulum vesicles, deduced from location studies of protein-bound spin labels.从蛋白质结合自旋标记的定位研究推导肌浆网囊泡中的钙转运机制。
Proc Natl Acad Sci U S A. 1980 May;77(5):2405-9. doi: 10.1073/pnas.77.5.2405.
7
Diffusion of a small molecule in the cytoplasm of mammalian cells.小分子在哺乳动物细胞质中的扩散。
Proc Natl Acad Sci U S A. 1984 Jun;81(11):3414-8. doi: 10.1073/pnas.81.11.3414.
8
Diffusion in the aqueous compartment.在水相区室中的扩散。
J Cell Biol. 1984 Jul;99(1 Pt 2):180s-187s. doi: 10.1083/jcb.99.1.180s.
9
Amine and carboxylate spin probe permeability in red cells.
J Membr Biol. 1989 Jul;109(1):41-52. doi: 10.1007/BF01870789.
10
Low viscosity in the aqueous domain of cell cytoplasm measured by picosecond polarization microfluorimetry.通过皮秒偏振显微荧光法测量细胞质水相区域的低粘度。
J Cell Biol. 1991 Feb;112(4):719-25. doi: 10.1083/jcb.112.4.719.
本文引用的文献
1
Rapid lateral diffusion of phospholipids in rabbit sarcoplasmic reticulum.磷脂在兔肌浆网中的快速侧向扩散。
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2056-60. doi: 10.1073/pnas.69.8.2056.
2
Viscosity of cellular protoplasm.细胞原生质的黏度
Science. 1974 Feb 15;183(4125):666-8. doi: 10.1126/science.183.4125.666.
3
Spin exchange of spin labeled probes in a natural membrane.
Biochem Biophys Res Commun. 1972 Sep 26;48(6):1362-6. doi: 10.1016/0006-291x(72)90862-5.
4
Studies of the crystalline-liquid crystalline phase transition of lipid model membranes. 3. Structure of a steroid-lecithin system below and above the lipid-phase transition.脂质模型膜的晶态-液晶态相变研究。3. 脂质相变温度上下的类固醇-卵磷脂体系结构。
J Am Chem Soc. 1972 Jun 28;94(13):4499-510. doi: 10.1021/ja00768a015.
5
Studies of the crystalline-liquid crystalline phase transition of lipid model membranes. II. Analysis of electron spin resonance spectra of steroid labels incorporated into lipid membranes.脂质模型膜的晶态-液晶态相变研究。II. 掺入脂质膜的类固醇标记物的电子自旋共振光谱分析。
J Am Chem Soc. 1972 Jun 28;94(13):4492-8. doi: 10.1021/ja00768a014.
6
Studies of the crystalline-liquid crystalline phase transition of lipid model membranes. I. Use of spin labels and optical probes as indicators of the phase transition.脂质模型膜的晶态-液晶态相变研究。I. 使用自旋标记和光学探针作为相变的指示剂。
J Am Chem Soc. 1972 Jun 28;94(13):4482-91. doi: 10.1021/ja00768a013.
7
Lateral diffusion in spin-labeled phosphatidylcholine multilayers.自旋标记磷脂酰胆碱多层膜中的横向扩散
J Am Chem Soc. 1972 Jun 28;94(13):4475-81. doi: 10.1021/ja00768a600.
8
Spin-labeled Neurospora mitochondria.自旋标记的粗糙脉孢菌线粒体。
Biophys J. 1970 Jul;10(7):618-29. doi: 10.1016/S0006-3495(70)86324-X.
Zotero 插件