Guttman D S, Wang P W, Wang I N, Bosler E M, Luft B J, Dykhuizen D E
Department of Ecology and Evolution, State University of New York at Stony Brook, USA.
J Clin Microbiol. 1996 Mar;34(3):652-6. doi: 10.1128/jcm.34.3.652-656.1996.
The genetic heterogeneity of the spirochete Borrelia burgdorferi within single adult black-legged ticks from Shelter Island, N.Y., was determined by cold, single-strand conformation polymorphism (SSCP) analysis. The central region of the ospA gene of B. burgdorferi from infected ticks was amplified by nested PCR. Amplified product of the correct size was obtained from 20 to 45 ticks (44%). This is the fraction of ticks that is expected to be infected with B. burgdorferi. Four variant classes were determined by SSCP analysis. Eight ticks were infected with a single variant, nine ticks were infected with two variants, two ticks were infected with three variants, and one tick was infected with all four variants. DNA from each variant was sequenced. Five different sequences were found. The sequence of each variant was different from that of another variant by a single base. SSCP analysis could distinguish three of the four single-base changes found in the region.
通过冷单链构象多态性（SSCP）分析，确定了来自纽约州谢尔特岛单个成年黑脚蜱体内伯氏疏螺旋体的遗传异质性。通过巢式PCR扩增受感染蜱中伯氏疏螺旋体ospA基因的中心区域。从20至45只蜱（44%）中获得了正确大小的扩增产物。这是预计感染伯氏疏螺旋体的蜱的比例。通过SSCP分析确定了四个变异类别。八只蜱感染了单一变异体，九只蜱感染了两种变异体，两只蜱感染了三种变异体，一只蜱感染了所有四种变异体。对每个变异体的DNA进行了测序。发现了五种不同的序列。每个变异体的序列与另一个变异体的序列仅相差一个碱基。SSCP分析可以区分该区域发现的四个单碱基变化中的三个。
