通过细胞培养-聚合酶链反应综合方法检测传染性肠道病毒
Detection of infectious enteroviruses by an integrated cell culture-PCR procedure.
作者信息
Reynolds K A, Gerba C P, Pepper I L
机构信息
Department of Soil, Water and Environmental Science, University of Arizona, Tucson 85721, USA.
出版信息
Appl Environ Microbiol. 1996 Apr;62(4):1424-7. doi: 10.1128/aem.62.4.1424-1427.1996.
Abstract
Rapid detection of infectious enteroviruses in environmental samples was made possible by utilizing an integrated cell culture-reverse transcriptase PCR approach. By this method, the presence of infectious enterovirus was confirmed within 24 h, compared with > or = 3 days by cell culture alone. The combined methodology eliminated typical problems normally associated with direct reverse transcriptase PCR by increasing the equivalent volume of environmental sample examined and reducing the effects of inhibitory compounds.
摘要
通过采用细胞培养-逆转录聚合酶链反应（PCR）综合方法，可快速检测环境样本中的传染性肠道病毒。采用这种方法，可在24小时内确认传染性肠道病毒的存在，而仅通过细胞培养法则需要3天或更长时间。该联合方法通过增加所检测环境样本的等效体积并减少抑制性化合物的影响，消除了通常与直接逆转录PCR相关的典型问题。
相似文献
1
Detection of infectious enteroviruses by an integrated cell culture-PCR procedure.通过细胞培养-聚合酶链反应综合方法检测传染性肠道病毒
Appl Environ Microbiol. 1996 Apr;62(4):1424-7. doi: 10.1128/aem.62.4.1424-1427.1996.
2
Evaluation of integrated cell culture-PCR (C-PCR) for virological analysis of environmental samples.用于环境样本病毒学分析的集成细胞培养 - 聚合酶链反应（C-PCR）评估
J Appl Microbiol. 2002;93(5):745-50. doi: 10.1046/j.1365-2672.2002.01741.x.
3
Environmental surveillance of enterovirus in Northern India using an integrated shell vial culture with a semi-nested RT PCR and partial sequencing of the VP1 gene.采用壳瓶培养与半巢式 RT-PCR 联合检测和 VP1 基因部分测序方法对印度北部肠道病毒进行环境监测。
J Med Virol. 2013 Mar;85(3):505-11. doi: 10.1002/jmv.23441.
4
Integrated cell culture/PCR for detection of enteric viruses in environmental samples.用于检测环境样本中肠道病毒的集成细胞培养/聚合酶链反应法
Methods Mol Biol. 2004;268:69-78. doi: 10.1385/1-59259-766-1:069.
5
Reverse transcription multiplex PCR for differentiation between polio- and enteroviruses from clinical and environmental samples.用于区分临床和环境样本中脊髓灰质炎病毒和肠道病毒的逆转录多重聚合酶链反应
J Clin Microbiol. 1995 Jun;33(6):1442-7. doi: 10.1128/jcm.33.6.1442-1447.1995.
6
[Evaluation of a new technology for the detection of enterovirus in sewage].[一种用于检测污水中肠道病毒的新技术的评估]
Rev Cubana Med Trop. 1999 Sep-Dec;51(3):166-71.
7
Detection of astroviruses, enteroviruses, and adenovirus types 40 and 41 in surface waters collected and evaluated by the information collection rule and an integrated cell culture-nested PCR procedure.通过信息收集规则以及集成细胞培养-巢式聚合酶链反应程序，对采集并评估的地表水中星状病毒、肠道病毒以及40型和41型腺病毒进行检测。
Appl Environ Microbiol. 2000 Jun;66(6):2520-5. doi: 10.1128/AEM.66.6.2520-2525.2000.
8
Incidence of enteroviruses in Mamala Bay, Hawaii using cell culture and direct polymerase chain reaction methodologies.采用细胞培养和直接聚合酶链反应方法检测夏威夷马马拉湾肠道病毒的发病率。
Can J Microbiol. 1998 Jun;44(6):598-604.
9
Rapid and sensitive detection of enteroviruses in specimens from patients with aseptic meningitis.无菌性脑膜炎患者标本中肠道病毒的快速灵敏检测
J Clin Microbiol. 1996 Jan;34(1):199-201. doi: 10.1128/jcm.34.1.199-201.1996.
10
Methods to remove inhibitors in sewage and other fecal wastes for enterovirus detection by the polymerase chain reaction.通过聚合酶链反应检测肠道病毒时去除污水及其他粪便废物中抑制剂的方法。
J Virol Methods. 1995 Jul;54(1):51-66. doi: 10.1016/0166-0934(95)00025-p.
引用本文的文献
1
Enteroviruses in Water: Epidemiology, Detection and Inactivation.水中肠道病毒：流行病学、检测与灭活
Environ Microbiol. 2025 May;27(5):e70109. doi: 10.1111/1462-2920.70109.
2
A review on the contamination of SARS-CoV-2 in water bodies: Transmission route, virus recovery and recent biosensor detection techniques.水体中新型冠状病毒污染的综述：传播途径、病毒回收及近期生物传感器检测技术
Sens Biosensing Res. 2022 Jun;36:100482. doi: 10.1016/j.sbsr.2022.100482. Epub 2022 Mar 2.
3
Chapter 9 The Detection of Waterborne Viruses.第9章 水传播病毒的检测
Perspect Med Virol. 2007;17:177-204. doi: 10.1016/S0168-7069(07)17009-9. Epub 2007 Sep 6.
4
Assessing the Occurrence of Waterborne Viruses in Reuse Systems: Analytical Limits and Needs.评估再生水系统中水源性病毒的发生情况：分析限度与需求
Pathogens. 2019 Jul 22;8(3):107. doi: 10.3390/pathogens8030107.
5
Overview of Trends in the Application of Metagenomic Techniques in the Analysis of Human Enteric Viral Diversity in Africa's Environmental Regimes.非洲环境条件下宏基因组技术在分析人类肠道病毒多样性中的应用趋势概述。
Viruses. 2018 Aug 14;10(8):429. doi: 10.3390/v10080429.
6
Temporary carriage of bovine coronavirus and bovine respiratory syncytial virus by fomites and human nasal mucosa after exposure to infected calves.接触感染小牛后，牛冠状病毒和牛呼吸道合胞病毒在污染物及人鼻黏膜上的短暂携带情况。
BMC Vet Res. 2018 Jan 22;14(1):22. doi: 10.1186/s12917-018-1335-1.
7
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR.美国环境保护局方法1615。通过培养和逆转录定量聚合酶链反应测定水中肠道病毒和诺如病毒的存在情况。第三部分。通过逆转录定量聚合酶链反应检测病毒
J Vis Exp. 2016 Jan 16(107):e52646. doi: 10.3791/52646.
8
New approaches for enhanced detection of enteroviruses from Hawaiian environmental waters.从夏威夷环境水中增强检测肠道病毒的新方法。
PLoS One. 2012;7(5):e32442. doi: 10.1371/journal.pone.0032442. Epub 2012 May 2.
9
Detection and quantification of poliovirus infection using FTIR spectroscopy and cell culture.利用傅里叶变换红外光谱和细胞培养检测和定量脊髓灰质炎病毒感染。
J Biol Eng. 2011 Dec 5;5:16. doi: 10.1186/1754-1611-5-16.
10
Virus hazards from food, water and other contaminated environments.食物、水和其他受污染环境中的病毒危害。
FEMS Microbiol Rev. 2012 Jul;36(4):786-814. doi: 10.1111/j.1574-6976.2011.00306.x. Epub 2011 Oct 24.
本文引用的文献
1
Increased sensitivity of poliovirus detection in tap water concentrates by reverse transcriptase-polymerase chain reaction.通过逆转录聚合酶链反应提高自来水中脊髓灰质炎病毒浓缩物检测的灵敏度。
J Virol Methods. 1995 Nov;55(3):295-302. doi: 10.1016/0166-0934(95)00065-6.
2
A method to detect enteroviruses in sewage sludge-amended soil using the PCR.一种利用聚合酶链反应（PCR）检测经污水污泥改良土壤中肠道病毒的方法。
Appl Environ Microbiol. 1994 Mar;60(3):1014-7. doi: 10.1128/aem.60.3.1014-1017.1994.
3
Detection of enteroviruses in groundwater with the polymerase chain reaction.用聚合酶链反应检测地下水中的肠道病毒。
Appl Environ Microbiol. 1993 May;59(5):1318-24. doi: 10.1128/aem.59.5.1318-1324.1993.
4
Detection of naturally occurring enteroviruses in waters by reverse transcription, polymerase chain reaction, and hybridization.通过逆转录、聚合酶链反应和杂交技术检测水中自然存在的肠道病毒。
Appl Environ Microbiol. 1993 Apr;59(4):1213-9. doi: 10.1128/aem.59.4.1213-1219.1993.
5
Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR.通过逆转录-聚合酶链反应检测水样中肠道病毒、甲型肝炎病毒和诺如病毒时，对牛肉提取物模拟洗脱液进行浓缩和纯化。
Appl Environ Microbiol. 1995 Feb;61(2):531-7. doi: 10.1128/aem.61.2.531-537.1995.
6
Simple method of concentrating enteroviruses and hepatitis A virus from sewage and ocean water for rapid detection by reverse transcriptase-polymerase chain reaction.从污水和海水中浓缩肠道病毒和甲型肝炎病毒以通过逆转录聚合酶链反应进行快速检测的简单方法。
Appl Environ Microbiol. 1993 Oct;59(10):3488-91. doi: 10.1128/aem.59.10.3488-3491.1993.
7
Polymerase chain reaction-gene probe detection of microorganisms by using filter-concentrated samples.使用过滤浓缩样本通过聚合酶链反应-基因探针检测微生物
Appl Environ Microbiol. 1991 Dec;57(12):3529-34. doi: 10.1128/aem.57.12.3529-3534.1991.
8
Rapid method for processing soil samples for polymerase chain reaction amplification of specific gene sequences.用于特定基因序列聚合酶链反应扩增的土壤样本快速处理方法。
Appl Environ Microbiol. 1991 Aug;57(8):2283-6. doi: 10.1128/aem.57.8.2283-2286.1991.
9
Presence of human immunodeficiency virus nucleic acids in wastewater and their detection by polymerase chain reaction.废水中人类免疫缺陷病毒核酸的存在及其通过聚合酶链反应进行的检测。
Appl Environ Microbiol. 1992 Dec;58(12):3984-90. doi: 10.1128/aem.58.12.3984-3990.1992.
10
Concentration of enteroviruses from large volumes of tap water, treated sewage, and seawater.从大量自来水、处理后的污水和海水中浓缩肠道病毒。
Appl Environ Microbiol. 1978 Mar;35(3):540-8. doi: 10.1128/aem.35.3.540-548.1978.
Zotero 插件