Kitajima H, Sumida Y, Tanaka R, Yuki N, Takayama H, Fujimura M
Osaka Medical Center, Japan.
Arch Dis Child Fetal Neonatal Ed. 1997 Mar;76(2):F101-7. doi: 10.1136/fn.76.2.f101.
To investigate the colonisation with Bifidobacterium breve of the bowels of very low birthweight (VLBW) infants.
The adverse effects of B breve were examined in 66 VLBW infants (preliminary study). A prospective randomised clinical study of 91 VLBW infants was also completed and these infants were followed up for three years. Precise viable bacterial counts of serial stool specimens were examined for the first eight weeks after birth in 10 infants. The colonisation rates of administered bacteria were examined using immunohistochemical staining of stool specimens with a B breve specific monoclonal antibody.
In the preliminary study there were no side effects attributable to the bacteria. Immunohistochemical staining of stool specimens showed that the colonisation rates of the administered bacteria were 73% at 2 weeks of age, but only 12% in the control group. Early administration of B breve significantly decreased aspirated air volume from the stomach and improved weight gain.
B breve can colonise the immature bowel very effectively and is associated with fewer abnormal abdominal signs and better weight gain in VLBW infants, probably as a result of stabilisation of their intestinal flora and accelerated feeding schedules.
研究极低出生体重（VLBW）婴儿肠道中短双歧杆菌的定植情况。
在66例极低出生体重婴儿中检测了短双歧杆菌的不良反应（初步研究）。还完成了一项对91例极低出生体重婴儿的前瞻性随机临床研究，并对这些婴儿进行了三年的随访。对10例婴儿出生后头八周的系列粪便标本进行了精确的活菌计数。使用短双歧杆菌特异性单克隆抗体对粪便标本进行免疫组织化学染色，检测所给予细菌的定植率。
在初步研究中，未发现与该细菌相关的副作用。粪便标本的免疫组织化学染色显示，给予细菌的定植率在2周龄时为73%，而对照组仅为12%。早期给予短双歧杆菌可显著减少胃内吸出气体量并改善体重增加。
短双歧杆菌能非常有效地在未成熟肠道定植，且与极低出生体重婴儿腹部异常体征较少和体重增加较好有关，这可能是其肠道菌群稳定和喂养计划加快的结果。
