Gurfield A N, Boulouis H J, Chomel B B, Heller R, Kasten R W, Yamamoto K, Piemont Y
Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, 95616, USA.
J Clin Microbiol. 1997 Aug;35(8):2120-3. doi: 10.1128/jcm.35.8.2120-2123.1997.
Bartonella clarridgeiae and several strains of Bartonella henselae, the agent of cat scratch disease, with variations in the 16S rRNA gene have been found to infect the blood of cats. An epidemiologic study of Bartonella infection in domestic French cats revealed that of 436 cats sampled, 5 cats (1.1%) were coinfected with B. henselae and B. clarridgeiae and 2 cats (0.5%) were coinfected with two strains of B. henselae with variations in the 16S rRNA gene, B. henselae type I and type II. In an indirect immunofluorescence assay, coinfected cats tested positive for both Bartonella species at titers of > or = 128. Identification of the colonies was achieved by preformed enzyme analysis, PCR-restriction fragment length polymorphism analysis of the citrate synthase gene, and 16S rRNA gene sequencing. Colony size differences in mixed culture allowed differentiation of the Bartonella species. The coinfection of cats with two Bartonella species or variants of the same species raises concern about the possibility of dual infection in humans. The development of a polyvalent vaccine targeted against the most pathogenic or invasive strains may be a means of protecting cats and man from infection.
巴尔通体克拉里奇亚种以及作为猫抓病病原体的数株汉赛巴尔通体，其16S rRNA基因存在变异，已被发现可感染猫的血液。一项针对法国家养猫巴尔通体感染的流行病学研究显示，在抽样的436只猫中，有5只（1.1%）同时感染了汉赛巴尔通体和克拉里奇巴尔通体，2只（0.5%）同时感染了16S rRNA基因存在变异的两株汉赛巴尔通体，即汉赛巴尔通体I型和II型。在间接免疫荧光试验中，同时感染的猫对两种巴尔通体的检测滴度均≥128时呈阳性。通过预制酶分析、柠檬酸合酶基因的聚合酶链反应-限制性片段长度多态性分析以及16S rRNA基因测序实现了菌落鉴定。混合培养中菌落大小的差异有助于区分巴尔通体菌种。猫同时感染两种巴尔通体菌种或同一菌种的变种引发了人们对人类双重感染可能性的担忧。研发针对最具致病性或侵袭性菌株的多价疫苗可能是保护猫和人类免受感染的一种手段。
