荷兰家猫中巴尔通体属物种的流行情况。
Prevalence of Bartonella species in domestic cats in The Netherlands.
作者信息
Bergmans A M, de Jong C M, van Amerongen G, Schot C S, Schouls L M
机构信息
Research Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, Bilthoven, The Netherlands.
出版信息
J Clin Microbiol. 1997 Sep;35(9):2256-61. doi: 10.1128/jcm.35.9.2256-2261.1997.
Cats have been shown to provide the only known reservoir of Bartonella henselae, the causative agent of cat scratch disease. To determine the prevalence of Bartonella bacteremia and antibodies in Dutch cats, blood samples from 113 cats from shelters (sheltered cats), 50 pet cats, and 25 specific-pathogen-free (SPF) cats were analyzed. Culture and subsequent PCR-restriction fragment length polymorphism (RFLP) analysis of the 16S-23S rRNA intergenic region and 16S rRNA gene PCR-hybridization assays revealed a prevalence of Bartonella bacteremia in 22% of the sheltered cats and showed no bacteremia in the SPF cats. Three spacer RFLP types were found: types A, B, and G, with type B being predominant over types A and G. An important finding was the existence of mixtures of different Bartonella species. Bartonella DNA was detected in 7 of 27 DNA extracts from fleas combed from the sheltered cats (26%). Seropositivity was 50% for sheltered cats and 56% for pet cats, as determined by a B. henselae enzyme-linked immunoassay.
猫已被证明是汉赛巴尔通体的唯一已知宿主，汉赛巴尔通体是猫抓病的病原体。为了确定荷兰猫中巴尔通体菌血症和抗体的流行情况，对来自收容所的113只猫（收容猫）、50只宠物猫和25只无特定病原体（SPF）猫的血样进行了分析。对16S - 23S rRNA基因间隔区进行培养及随后的PCR - 限制性片段长度多态性（RFLP）分析，以及16S rRNA基因PCR - 杂交检测显示，22%的收容猫存在巴尔通体菌血症，而SPF猫未出现菌血症。发现了三种间隔RFLP类型：A、B和G型，其中B型占主导地位，超过A和G型。一个重要发现是存在不同巴尔通体物种的混合情况。在从收容猫身上梳下的27只跳蚤的DNA提取物中，有7只（26%）检测到巴尔通体DNA。通过汉赛巴尔通体酶联免疫测定法测定，收容猫的血清阳性率为50%，宠物猫为56%。
Zotero 插件