Yokota S, Amano K, Hayashi S, Fujii N
Sumitomo Pharmaceuticals Research Center, Osaka, Japan.
Infect Immun. 1997 Sep;65(9):3509-12. doi: 10.1128/iai.65.9.3509-3512.1997.
We have examined the antibody response to Helicobacter pylori lipopolysaccharide (LPS) during natural infection in humans. The sera of over 70% of H. pylori-infected individuals were found to contain immunoglobulin G antibodies against the LPS fractions isolated from smooth strains of H. pylori but not against those derived from rough strains, as determined by enzyme-linked immunosorbent assay. These results taken together with the immunoblot data indicated that the polysaccharide region of H. pylori LPS is antigenic in humans. However, the antigenicity of the polysaccharide varied, depending on the strain. We found that smooth H. pylori strains isolated from the tumors of patients with gastric cancer showed significantly lower antigenicity than smooth strains derived from patients with chronic gastritis and gastric and duodenal ulcers. The results suggest that the levels of antigenicity of the polysaccharide region of H. pylori LPS in humans correlate with the nature of the gastroduodenal diseases and that they allow a particular distinction to be made between gastric cancer and other gastroduodenal diseases, especially chronic gastritis.
我们研究了人类自然感染幽门螺杆菌脂多糖（LPS）后的抗体反应。通过酶联免疫吸附测定法确定，超过70%的幽门螺杆菌感染个体的血清中含有针对从幽门螺杆菌光滑菌株分离的LPS组分的免疫球蛋白G抗体，但不含有针对从粗糙菌株衍生的LPS组分的抗体。这些结果与免疫印迹数据一起表明，幽门螺杆菌LPS的多糖区域在人类中具有抗原性。然而，多糖的抗原性因菌株而异。我们发现，从胃癌患者肿瘤中分离出的光滑幽门螺杆菌菌株的抗原性明显低于从慢性胃炎及胃和十二指肠溃疡患者中分离出的光滑菌株。结果表明，人类幽门螺杆菌LPS多糖区域的抗原性水平与胃十二指肠疾病的性质相关，并且它们能够在胃癌与其他胃十二指肠疾病，尤其是慢性胃炎之间做出特定区分。
