8-甲氧基补骨脂素与紫外线A体外处理后的白细胞趋化性。

Leukocyte chemotaxis after in vitro treatment with 8-methoxypsoralen and UVA.

作者信息

Langner A, Christophers E

出版信息

Arch Dermatol Res (1975). 1977 Oct 27;260(1):51-5. doi: 10.1007/BF00558014.

Abstract

Isolated human polymorphonuclear leukocytes were submitted to long wave ultraviolet light (UVA) with and without preincubation of the cells with 8-methoxypsoralen (8-MOP). Leukocyte chemotaxis was determined in modified Boyden chambers using caseine as the attractant. Combined treatment (8-MOP + UVA) significantly inhibited the chemotactic activity with 8-MOP concentrations above 0.1 mug/ml and 2 J/cm(2). However, with high dosage of UVA (5 J/cm(2)) with and without 8-MOP still 25-30% cells migrated through the filters. Also, cell viability as determined by trypan blue exclusion was only moderately affected by combined treatment. The results indicate that these nonreplicating cells are comperatively insensitive to UVA and 8-MOP.

摘要

将分离出的人多形核白细胞暴露于长波紫外线（UVA）下，细胞分别在有和没有用8-甲氧基补骨脂素（8-MOP）预孵育的情况下接受照射。在改良的博伊登小室中，以酪蛋白作为趋化剂来测定白细胞趋化性。当8-MOP浓度高于0.1μg/ml且紫外线剂量为2J/cm²时，联合治疗（8-MOP + UVA）显著抑制了趋化活性。然而，在有和没有8-MOP的情况下，当紫外线剂量较高（5J/cm²）时，仍有25%-30%的细胞穿过滤膜迁移。此外，通过台盼蓝拒染法测定的细胞活力仅受到联合治疗的中度影响。结果表明，这些不再增殖的细胞对UVA和8-MOP相对不敏感。