Gallardo M E, Ferrández A, De Lorenzo V, García J L, Díaz E
Departamento de Microbiología Molecular, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
J Bacteriol. 1997 Nov;179(22):7156-60. doi: 10.1128/jb.179.22.7156-7160.1997.
The dsz biodesulfurization cluster from Rhodococcus erythropolis IGTS8 has been engineered under the control of heterologous broad-host-range regulatory signals to alleviate the mechanism of sulfur repression, and it was stably inserted into the chromosomes of different Pseudomonas strains. The recombinant bacteria were able to desulfurize dibenzothiophene more efficiently than the native host. Furthermore, these new biocatalysts combine relevant industrial and environmental traits, such as production of biosurfactants, with the enhanced biodesulfurization phenotype.
来自红平红球菌IGTS8的dsz生物脱硫基因簇已在异源广宿主范围调控信号的控制下进行改造，以缓解硫抑制机制，并稳定插入不同假单胞菌菌株的染色体中。重组菌比天然宿主更有效地对二苯并噻吩进行脱硫。此外，这些新型生物催化剂将相关的工业和环境特性（如生物表面活性剂的产生）与增强的生物脱硫表型相结合。
