Mättö J, Saarela M, Alaluusua S, Oja V, Jousimies-Somer H, Asikainen S
Research Laboratory, Institute of Dentistry, University of Helsinki, Finland.
J Clin Microbiol. 1998 Jan;36(1):157-60. doi: 10.1128/JCM.36.1.157-160.1998.
Simple sample-processing methods for PCR detection of Porphyromonas gingivalis, a major pathogen causing adult periodontitis, from saliva were studied. The ability to detect P. gingivalis from 118 salivary samples by PCR after boiling and Chelex 100 processing was compared with bacterial culture. P. gingivalis was detected three times more often by PCR than by culture. Chelex 100 processing of saliva proved to be effective in preventing PCR inhibition and was applied to determine the occurrence of P. gingivalis in saliva samples from 263 Finnish subjects between 5 and 80 years of age. The occurrence of P. gingivalis increased with age, and it was detected by PCR in the saliva of 5.0% of subjects between 5 and 10 years of age, 13.8% of subjects between 11 and 20 years of age, 13.4% of subjects between 21 and 30 years of age, and 63.3% of subjects between 31 and 80 years of age. The results indicate that P. gingivalis is a rare finding in saliva from periodontally healthy children and young adults but a frequent one in saliva from adult periodontitis patients.
研究了用于从唾液中PCR检测牙龈卟啉单胞菌（一种导致成人牙周炎的主要病原体）的简单样本处理方法。将煮沸和Chelex 100处理后通过PCR从118份唾液样本中检测牙龈卟啉单胞菌的能力与细菌培养法进行了比较。通过PCR检测到牙龈卟啉单胞菌的频率是培养法的三倍。事实证明，Chelex 100处理唾液可有效防止PCR抑制，并用于确定263名年龄在5至80岁之间的芬兰受试者唾液样本中牙龈卟啉单胞菌的存在情况。牙龈卟啉单胞菌的存在情况随年龄增长而增加，在5至10岁的受试者中，5.0%的人唾液中通过PCR检测到该菌；在11至20岁的受试者中，13.8%的人检测到；在21至30岁的受试者中，13.4%的人检测到；在31至80岁的受试者中，63.3%的人检测到。结果表明，牙龈卟啉单胞菌在牙周健康的儿童和年轻人的唾液中很少见，但在成人牙周炎患者的唾液中很常见。
