人β干扰素基因在体细胞杂种中的定位与表达

Human beta interferon gene localization and expression in somatic cell hybrids.

作者信息

Pitha P M, Slate D L, Raj N B, Ruddle F H

出版信息

Mol Cell Biol. 1982 May;2(5):564-70. doi: 10.1128/mcb.2.5.564-570.1982.

DOI:10.1128/mcb.2.5.564-570.1982

PMID:6180309

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC369826/

Abstract

The human fibroblast interferon gene beta 1 was mapped to human chromosome 9. Sequence homology with a beta 1 cDNA clone was detected in both genomic DNA and induced mRNA of human/mouse or human/hamster somatic cell hybrids containing human chromosome 9, but not in lines lacking this chromosome or those retaining a complex translocation involving chromosomes 9 and 11. Interferon mRNA that did not share sequence homology with the beta 1 cDNA clone was detected in lines containing human chromosomes 2 and 5 but lacking chromosome 9, suggesting the presence of other unlinked interferon sequences in the human genome.

摘要

人类成纤维细胞干扰素基因β1被定位到人类9号染色体上。在含有人类9号染色体的人/鼠或人/仓鼠体细胞杂种的基因组DNA和诱导的mRNA中，均检测到与β1 cDNA克隆的序列同源性，但在缺乏该染色体的细胞系或保留涉及9号和11号染色体复杂易位的细胞系中未检测到。在含有人类2号和5号染色体但缺乏9号染色体的细胞系中，检测到与β1 cDNA克隆不具有序列同源性的干扰素mRNA，这表明人类基因组中存在其他不连锁的干扰素序列。