Zeitlin L, Whaley K J, Hegarty T A, Moench T R, Cone R A
Department of Biophysics, Johns Hopkins University, Baltimore, Maryland 21218, USA.
Contraception. 1997 Nov;56(5):329-35. doi: 10.1016/s0010-7824(97)00154-6.
Microbicide candidates were selected that have demonstrated activity against sperm or sexually transmitted disease pathogens in vitro, and the efficacy of these agents for preventing vaginal transmission of genital herpes infection was evaluated in the progestin-treated mouse. Each agent was delivered to the vaginas of mice approximately 20 sec prior to delivering a highly infectious herpes simplex virus-2 inoculum. The following agents provided significant protection: anti-HSV monoclonal antibodies III-174 and HSV8, modified bovine beta-lactoglobulin (beta-69), carrageenan, concanavalin A, chlorhexidine, dextran sulfate (average molecular weight 8,000 and 500,000), fucoidan, neem, nonoxynol-9, polystyrene sulfonate, and povidone-iodine. Two agents, gramicidin and heparan sulfate, though highly effective in vitro, were not protective in vivo at the doses tested.
筛选出在体外已证明对精子或性传播疾病病原体有活性的杀微生物剂候选物，并在孕激素处理的小鼠中评估这些制剂预防阴道传播生殖器疱疹感染的功效。在接种高传染性单纯疱疹病毒2之前约20秒，将每种制剂递送至小鼠阴道。以下制剂提供了显著的保护作用：抗HSV单克隆抗体III-174和HSV8、修饰的牛β-乳球蛋白（β-69）、角叉菜胶、伴刀豆球蛋白A、洗必泰、硫酸葡聚糖（平均分子量8000和500000）、岩藻依聚糖、印楝、壬苯醇醚-9、聚苯乙烯磺酸盐和聚维酮碘。两种制剂，即短杆菌肽和硫酸乙酰肝素，尽管在体外非常有效，但在所测试的剂量下在体内没有保护作用。
