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通过巢式PCR扩增检测市游泳池中的腺病毒爆发。

Detection of adenovirus outbreak at a municipal swimming pool by nested PCR amplification.

作者信息

Papapetropoulou M, Vantarakis A C

机构信息

University of Patras Medical School, Public Health Laboratory, Environmental Microbiology Patras, Greece.

出版信息

J Infect. 1998 Jan;36(1):101-3. doi: 10.1016/s0163-4453(98)93414-4.

DOI:10.1016/s0163-4453(98)93414-4
PMID:9515677
Abstract

In July 1995 an outbreak of pharyngoconjuctivitis caused by adenoviruses occurred among athletes participating in a swimming contest in a town in southern Greece (Peloponnese). At least 80 persons displayed symptoms of the illness, with the predominant ones being high fever, sore throat, conjuctivitis, headache, and abdominal pain. Poor chlorination was probably the cause of the outbreak (residual chlorine <0.2 mg/l), as after hyperchlorination the spread of adenoviruses stopped. Rapid detection of adenoviruses in the municipal swimming pool water by nested polymerase chain reaction (PCR) amplification allowed quick control of the outbreak.

摘要

1995年7月,希腊南部(伯罗奔尼撒半岛)一个城镇的运动员参加游泳比赛期间,发生了一起由腺病毒引起的咽结膜热疫情。至少80人出现了该病症状,主要症状为高烧、喉咙痛、结膜炎、头痛和腹痛。氯化处理不当可能是疫情爆发的原因(余氯<0.2毫克/升),因为在进行强化氯化处理后,腺病毒的传播停止了。通过巢式聚合酶链反应(PCR)扩增快速检测市政游泳池水中的腺病毒,使得疫情能够迅速得到控制。

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