Vandamme A M, Salemi M, Van Brussel M, Liu H F, Van Laethem K, Van Ranst M, Michels L, Desmyter J, Goubau P
Rega Institute for Medical Research and University Hospitals, Katholieke Universiteit Leuven, Belgium.
J Virol. 1998 May;72(5):4327-40. doi: 10.1128/JVI.72.5.4327-4340.1998.
We identified a potential new subtype within human T-cell lymphotropic virus type 2 (HTLV-2), HTLV-2d, present in members of an isolated Efe Bambuti Pygmy tribe. Two of 23 Efe Pygmies were HTLV-2 seropositive, with HTLV-2 Western blot and enzyme-linked immunosorbent assay reactivities. From one of them the entire genome of the HTLV-2 strain Efe2 could be amplified and sequenced. In all gene regions analyzed, this strain was the most divergent HTLV-2 strain, differing by 2.4% (tax/rex) to 10.7% (long terminal repeat) from both subtypes HTLV-2a and HTLV-2b, yet major functional elements are conserved. The similarity between the HTLV-2 Efe2 Gag and Env proteins and the corresponding HTLV-2a and -2b proteins is consistent with the observed serological reactivity. In the proximal pX region, one of the two alternative splice acceptor sites is abolished in HTLV-2 Efe2. Another interesting feature of this potential new subtype is that it has a Tax protein of 344 amino acids (aa), which is intermediate in length between the HTLV-2a Tax protein (331 aa) and the HTLV-2b and -2c Tax proteins (356 aa) and similar to the simian T-cell lymphotropic virus type 2 (STLV-2) PP1664 Tax protein. Together these two findings suggest a different phenotype for the HTLV-2 Efe2 strain. Phylogenetic analyses confirmed that the Pygmy Efe2 strain potentially belonged to a new and quite divergent subtype, HTLV-2d. When the STLV-2 bonobo viruses PP1664 and PanP were used as an outgroup, it was clear that the Pygmy HTLV-2 Efe2 strain had the longest independent evolution and that HTLV-2 evolution is consistent with an African origin.
我们在人类嗜T细胞病毒2型（HTLV - 2）中鉴定出一种潜在的新亚型——HTLV - 2d，它存在于一个与世隔绝的埃菲·班布蒂俾格米部落成员中。23名埃菲俾格米人中有两人HTLV - 2血清学呈阳性，通过HTLV - 2免疫印迹法和酶联免疫吸附测定法呈现反应性。从其中一人身上能够扩增并测序HTLV - 2埃菲2株的全基因组。在所有分析的基因区域中，该毒株是分歧最大的HTLV - 2毒株，与HTLV - 2a和HTLV - 2b两个亚型相比，差异在2.4%（tax/rex）至10.7%（长末端重复序列）之间，但主要功能元件是保守的。HTLV - 2埃菲2株的Gag和Env蛋白与相应的HTLV - 2a和 - 2b蛋白之间的相似性与观察到的血清学反应性一致。在近端pX区域，HTLV - 2埃菲2株中两个可变剪接受体位点之一被消除。这种潜在新亚型的另一个有趣特征是它具有一个344个氨基酸（aa）的Tax蛋白，其长度介于HTLV - 2a Tax蛋白（331 aa）和HTLV - 2b及 - 2c Tax蛋白（356 aa）之间，并且与猿猴嗜T细胞病毒2型（STLV - 2）PP1664 Tax蛋白相似。这两个发现共同表明HTLV - 2埃菲2株具有不同的表型。系统发育分析证实，俾格米人埃菲2株可能属于一个新的且差异很大的亚型——HTLV - 2d。当将STLV - 2倭黑猩猩病毒PP1664和PanP用作外群时，很明显俾格米人HTLV - 2埃菲2株具有最长的独立进化历程，并且HTLV - 2的进化与非洲起源一致。
