Tapiovaara H, Olesen N J, Lindén J, Rimaila-Pärnänen E, von Bonsdorff C H
National Veterinary and Food Research Institute, Department of Virology and Epidemiology, Helsinki, Finland.
Dis Aquat Organ. 1998 Apr 3;32(3):185-93. doi: 10.3354/dao032185.
We have isolated a large virus from pike-perch Stizostedion lucioperca fingerlings with no signs of disease. The biochemical structural, and serological properties of this newly isolated virus suggest that it belongs to the family Iridoviridae. The virus multiplied and was cytopathogenic in several cultured fish cell lines. The virus has a DNA-containing genome and is assembled in the cytoplasm. When viewed in electron micrographs, the assembly sites showed a paracrystalline array of hexagonal nucleocapsids. The ultrastructure of the pike-perch virus resembled that of previously isolated fish iridoviruses. It is an enveloped icosahedral DNA virus. The diameter of the nucleocapsid in thin sections was 127 +/- 3 nm; in negatively stained preparates the size of the enveloped virus varied from 147 to 187 nm. In immunofluorescence the virus was stained by rabbit antisera against EHN (epizootic haematopoietic necrosis) virus, sheatfish iridovirus and cod iridovirus. The pathogenicity of the virus isolate was studied by inoculation into juvenile rainbow trout Oncorhyncus mykiss. Experimental infection under aquarium conditions suggested that the virus is apothogenic to rainbow trout. The infective virus could be recovered from the viscera of inoculated fish during the first week post-infection, after which the proportion of virus-positive fish declined over time. A small proportion of the fish still carried the virus 24 d post-inoculation.
我们从没有疾病迹象的梭鲈幼鱼中分离出一种大型病毒。这种新分离出的病毒的生化、结构和血清学特性表明它属于虹彩病毒科。该病毒能在几种培养的鱼类细胞系中增殖并具有细胞致病性。该病毒具有含DNA的基因组，在细胞质中装配。在电子显微镜照片中观察时，装配位点显示出六边形核衣壳的准晶体排列。梭鲈病毒的超微结构与先前分离出的鱼类虹彩病毒相似。它是一种有包膜的二十面体DNA病毒。薄切片中核衣壳的直径为127±3纳米；在负染制剂中，包膜病毒的大小在147至187纳米之间变化。在免疫荧光试验中，该病毒被抗EHN（流行性造血坏死）病毒、六须鲶虹彩病毒和鳕鱼虹彩病毒的兔抗血清染色。通过接种到幼年虹鳟Oncorhyncus mykiss中来研究该病毒分离株的致病性。水族箱条件下的实验感染表明该病毒对虹鳟无致病性。在感染后的第一周内可从接种鱼的内脏中分离到感染性病毒，此后病毒阳性鱼的比例随时间下降。接种后24天仍有一小部分鱼携带该病毒。
