de Vaux A, Laguerre G, Diviès C, Prévost H
Laboratoire de Microbiologie U. A. INRA, ENSBANA, Université de Bourgogne, Dijon, France.
Int J Syst Bacteriol. 1998 Apr;48 Pt 2:383-7. doi: 10.1099/00207713-48-2-383.
Several Gram-positive, non-spore-forming and non-motile bacteria consisting of pairs or chains of cocci were isolated during an investigation of the bacterial flora of the caecum of donkeys. Physiological and metabolic data indicated that the strains belong to the genus Enterococcus; phenotypic traits of these organisms were not consistent with any of the currently known Enterococcus species. 16S rRNA gene sequence analysis placed these strains in the genus Enterococcus. Their closest relatives are Enterococcus avium, Enterococcus faecium and Enterococcus pseudoavium with a sequence similarity of 97.4%. This group of strains can be differentiated from the other Enterococcus spp. by their phenotypic characteristics: strains do not grow in 6.5% NaCl; they do not produce acid from mannitol, sorbitol, sorbose, sucrose, raffinose, ribose and tagatose; they produce acid from D-xylose; they are able to utilize pyruvate; and they present a negative reaction on arginine. The name Enterococcus asini sp. nov. is proposed for these strains; the type strain is AS2T (= DSM 11492T).
在对驴盲肠细菌区系的调查中，分离出了几种革兰氏阳性、不形成芽孢且不运动的细菌，这些细菌由成对或成链的球菌组成。生理和代谢数据表明这些菌株属于肠球菌属；这些生物体的表型特征与目前已知的任何肠球菌物种均不一致。16S rRNA基因序列分析将这些菌株归入肠球菌属。它们最接近的亲缘种是鸟肠球菌、屎肠球菌和伪鸟肠球菌，序列相似性为97.4%。这组菌株可通过其表型特征与其他肠球菌属物种区分开来：菌株在6.5%氯化钠中不生长；它们不能从甘露醇、山梨醇、山梨糖、蔗糖、棉子糖、核糖和塔格糖产酸；它们能从D-木糖产酸；它们能够利用丙酮酸；并且它们对精氨酸呈阴性反应。提议将这些菌株命名为新种驴肠球菌；模式菌株为AS2T（=DSM 11492T）。
