Chou H P, Spence C, Scherer A, Quake S
Department of Applied Physics, California Institute of Technology, Pasadena, CA 91125, USA.
Proc Natl Acad Sci U S A. 1999 Jan 5;96(1):11-3. doi: 10.1073/pnas.96.1.11.
We have demonstrated a microfabricated single-molecule DNA sizing device. This device does not depend on mobility to measure molecule size, is 100 times faster than pulsed-field gel electrophoresis, and has a resolution that improves with increasing DNA length. It also requires a million times less sample than pulsed-field gel electrophoresis and has comparable resolution for large molecules. Here we describe the fabrication and use of the single-molecule DNA sizing device for sizing and sorting DNA restriction digests and ladders spanning 2-200 kbp.
我们展示了一种微制造的单分子DNA大小测定装置。该装置不依赖迁移率来测量分子大小，比脉冲场凝胶电泳快100倍，并且分辨率随着DNA长度的增加而提高。它所需的样品量比脉冲场凝胶电泳少一百万倍，并且对大分子具有相当的分辨率。在这里，我们描述了用于对跨度为2 - 200 kbp的DNA限制性消化产物和梯状条带进行大小测定和分选的单分子DNA大小测定装置的制造和使用。
