Yong D C, Prytula A
J Clin Microbiol. 1978 Dec;8(6):643-7. doi: 10.1128/jcm.8.6.643-647.1978.
A rapid carbohydrate utilization procedure for the confirmation of Neisseria gonorrhoeae and identification of other Neisseria species has been developed. This method utilizes both preformed enzymes, introduced in a heavy inoculum, and enzymes formed by the microorganisms as a result of growth in a small volume of super-enriched medium. Expected carbohydrate reactions were produced by 383 clinical isolates of neisseriae and were clearly visible within 4 h of incubation. The combined use of disposable glass tubes (6 by 50 mm) and microamounts of media (0.05 ml) make this method not only rapid, but also low in cost.
已开发出一种用于确认淋病奈瑟菌和鉴定其他奈瑟菌属菌种的快速碳水化合物利用程序。该方法利用了大量接种物中引入的预先形成的酶以及微生物在少量超富集培养基中生长所形成的酶。383株奈瑟菌临床分离株产生了预期的碳水化合物反应，并且在培养4小时内即可清晰观察到。一次性玻璃管（6×50毫米）和微量培养基（0.05毫升）的联合使用使得该方法不仅快速，而且成本低廉。
