Noguchi T, Jeon J K, Arakawa O, Sugita H, Deguchi Y, Shida Y, Hashimoto K
J Biochem. 1986 Jan;99(1):311-4. doi: 10.1093/oxfordjournals.jbchem.a135476.
Vibrio sp. isolated from a xanthid crab, Atergatis floridus, was cultured, and tetrodotoxin (TTX) and anhydroTTX were indicated to be present in several fractions of the cell extract and the culture medium by reverse phase HPLC. The presence of the C9-base in alkaline hydrolyzates of these fractions was confirmed by GC-MS and UV spectrometry. These results showed the production of TTX and anhydroTTX in the Vibrio sp., thus indicating one of the origins of TTX in nature.
从黄斑蟹（Atergatis floridus）分离出的弧菌属细菌进行培养后，通过反相高效液相色谱法表明，细胞提取物和培养基的几个组分中存在河豚毒素（TTX）和脱水河豚毒素。通过气相色谱 - 质谱联用仪和紫外光谱法确认了这些组分的碱性水解产物中存在C9碱。这些结果表明弧菌属细菌产生了TTX和脱水河豚毒素，从而揭示了自然界中TTX的来源之一。
