Lim C S, Kim S H, Kwon S I, Song J W, Song K J, Lee K N
Department of Clinical Pathology, Institute for Viral Diseases, College of Medicine, Korea University, Seoul, Republic of Korea.
Am J Trop Med Hyg. 2000 Feb;62(2):261-5. doi: 10.4269/ajtmh.2000.62.261.
The merozoite surface protein-1 (MSP-1) of Plasmodium vivax exhibits great antigenic diversity among different isolates of this parasite. This antigen is a useful genetic marker for studying the polymorphism of natural P. vivax parasite populations. One or more of these populations has been responsible for resurgent malaria now occurring in Korea. This paper reports the analysis of a highly polymorphic region between interspecies conserved blocks 5 and 6 of the MSP-1 gene, using the polymerase chain reaction to amplify the DNA fragment encompassing these regions from 25 Korean isolates, followed by sequencing. Almost all amino acid sequences of Korean isolates were nearly identical to that of Thai isolates TD525A (96.6-99.7%) and TD424 (96.3-99.5%), and very similar to that of the France-Belem strain when compared with other isolates (Sal-1, Sri Lanka, and Colombia). Interallelic recombination was found in the poly-Q repeat and a Sal-1 type amino acid structure was observed in all isolates. This study shows that the MSP gene nucleotide sequence of resurgent P. vivax in Korea is most similar to that of Thai isolates; however, the Korean strains are phylogenetically unique.
间日疟原虫的裂殖子表面蛋白-1（MSP-1）在该寄生虫的不同分离株中表现出极大的抗原多样性。这种抗原是研究间日疟原虫自然种群多态性的有用遗传标记。这些种群中的一个或多个是韩国目前复发性疟疾的病因。本文报道了对MSP-1基因种间保守区5和6之间高度多态性区域的分析，使用聚合酶链反应从25个韩国分离株中扩增包含这些区域的DNA片段，随后进行测序。韩国分离株的几乎所有氨基酸序列与泰国分离株TD525A（96.6 - 99.7%）和TD424（96.3 - 99.5%）的序列几乎相同，与其他分离株（Sal-1、斯里兰卡和哥伦比亚）相比，与法国-贝伦菌株的序列非常相似。在多聚Q重复序列中发现了等位基因间重组，并且在所有分离株中都观察到了Sal-1型氨基酸结构。这项研究表明，韩国复发性间日疟原虫的MSP基因核苷酸序列与泰国分离株的序列最相似；然而，韩国菌株在系统发育上是独特的。
