Bouchard M, Pinsonneault L, Tremblay C, Weber J P
Département de santé environnementale et santé au travail, Faculté de medecine, University of Montreal, Montreal, Quebec, Canada.
Int Arch Occup Environ Health. 2001 Sep;74(7):505-13. doi: 10.1007/s004200100251.
This study was undertaken to evaluate the environmental exposure to polycyclic aromatic hydrocarbons (PAHs) in nonsmoking adult subjects living in the vicinity of a creosote impregnation plant in Delson, Canada. Urinary metabolites of naphthalene, alpha- and beta-naphthol, and pyrene metabolite 1-hydroxypyrene (1-OHP), were used as biomarkers of exposure.
Morning and evening urine samples were collected in mid-August from 30 exposed individuals living at a distance of 50-360 m downwind of the plant and from a control group in the adjoining municipality residing at a distance of 1.9-2.7 km upwind of the plant. Metabolites were measured by gas chromatography/ mass spectrometry.
Excretion values of alpha- and beta-naphthol were significantly higher in the exposed group than in controls (P < 0.04), after accounting for possible confounding variables by multivariate analyses. The respective geometric mean concentrations (5th and 95th percentiles) of alpha-naphthol for the exposed and nonexposed groups were 2.04 (0.55-6.00) and 1.37 (0.39-7.02) micromol/mol creatinine for evening samples, and 2.49 (0.77-8.43) and 1.17 (0.37-6.88) micromol/mol creatinine for morning samples. Corresponding values for beta-naphthol were 1.78 (0.82-3.67) and 1.36 (0.63-5.07) micromol/mol creatinine for evening samples, and 1.94 (1.03-4.96) and 1.08 (0.49-5.05) micromol/mol creatinine for morning samples. On the other hand, no significant difference in 1-OHP excretion was observed between the exposed and the control group (P>0.5). The respective geometric mean concentrations (5th and 95th percentiles) of 1-OHP for these groups were 0.05 (0.01-0.17) and 0.06 (0.01-0.48) micromol/mol creatinine for evening samples, and 0.05 (0.02-0.12) and 0.05 (0.01-0.42) micromol/mol creatinine for morning samples.
The measurement of alpha- and beta-naphthol urinary concentrations appears to be an approach sufficiently sensitive to reveal differences in low exposure levels of volatile PAHs due to creosote impregnation plant emissions. However, uptake of pyrene due to the plant was too small to contribute significantly to 1-OHP excretion.
本研究旨在评估居住在加拿大德尔森一家杂酚油浸渍厂附近的非吸烟成年受试者的多环芳烃（PAHs）环境暴露情况。萘、α-和β-萘酚的尿代谢产物以及芘代谢产物1-羟基芘（1-OHP）被用作暴露生物标志物。
8月中旬，收集了30名暴露个体的晨尿和晚尿样本，这些个体居住在工厂下风方向50 - 360米处，同时还收集了毗邻市镇的一个对照组的尿样，该对照组居住在工厂上风方向1.9 - 2.7千米处。代谢产物通过气相色谱/质谱法进行测定。
在通过多变量分析考虑了可能的混杂变量后，暴露组中α-和β-萘酚的排泄值显著高于对照组（P < 0.04）。暴露组和非暴露组晚尿样本中α-萘酚的几何平均浓度（第5和第95百分位数）分别为2.04（0.55 - 6.00）和1.37（0.39 - 7.02）微摩尔/摩尔肌酐，晨尿样本分别为2.49（0.77 - 8.43）和1.17（0.37 - 6.88）微摩尔/摩尔肌酐。β-萘酚的相应值晚尿样本分别为1.78（0.82 - 3.67）和1.36（0.63 - 5.07）微摩尔/摩尔肌酐，晨尿样本分别为1.94（1.03 - 4.96）和1.08（0.49 - 5.05）微摩尔/摩尔肌酐。另一方面，暴露组和对照组之间未观察到1-OHP排泄的显著差异（P > 0.5）。这些组晚尿样本中1-OHP的几何平均浓度（第5和第95百分位数）分别为0.05（0.01 - 0.17）和0.06（0.01 - 0.48）微摩尔/摩尔肌酐，晨尿样本分别为0.05（0.02 - 0.12）和0.05（0.01 - 0.42）微摩尔/摩尔肌酐。
测量尿中α-和β-萘酚浓度似乎是一种足够敏感的方法，能够揭示由于杂酚油浸渍厂排放导致的挥发性PAHs低暴露水平的差异。然而，工厂导致的芘摄取量过小，对1-OHP排泄的贡献不显著。
