Fass R J, Barnishan J
Antimicrob Agents Chemother. 1979 Oct;16(4):434-8. doi: 10.1128/AAC.16.4.434.
The effects of supplementing Mueller-Hinton broth with calcium and magnesium on the minimal inhibitory concentrations (MICs) of eight aminoglycosides, colistin, tetracycline, and carbenicillin for 11 nonfermenters other than Pseudomonas aeruginosa were studied and compared with the effects for Escherichia coli and P. aeruginosa. MICs were simultaneously performed in unsupplemented Mueller-Hinton broth and Mueller-Hinton broth supplemented to contain 5 mg of calcium and 2.5 mg of magnesium per dl. Changes in MICs were expressed as the increases in the number of log(2) concentrations caused by supplementation. The usual increases in MICs of aminoglycosides caused by supplementation were: zero concentrations for E. coli, one to six concentrations for P. aeruginosa, and one to two concentrations for most other nonfermenters. The largest increases (five to six concentrations) were observed with gentamicin and P. aeruginosa. The usual increases in MICs of colistin were: zero concentrations for E. coli, two concentrations for P. aeruginosa, and one to two concentrations for other nonfermenters. Increases in MICs of tetracycline were: one to five concentrations for all organisms tested. The usual increases in MICs of carbenicillin were: zero concentrations for E. coli and P. aeruginosa and zero to two concentrations for other nonfermenters. These observations indicated that supplementation of Mueller-Hinton broth to contain recommended concentrations of calcium and magnesium had little effect on MICs of aminoglycosides and colistin for E. coli but increased MICs for most nonfermenters, increased MICs of tetracycline for E. coli and all nonfermenters, and had little effect on MICs of carbenicillin for E. coli and P. aeruginosa but increased the MICs for several nonfermenters other than P. aeruginosa.
研究了在穆勒-欣顿肉汤中添加钙和镁对除铜绿假单胞菌外的11种非发酵菌的8种氨基糖苷类、黏菌素、四环素和羧苄青霉素的最低抑菌浓度（MICs）的影响，并与对大肠杆菌和铜绿假单胞菌的影响进行了比较。在未添加钙和镁的穆勒-欣顿肉汤以及添加了每分升含5毫克钙和2.5毫克镁的穆勒-欣顿肉汤中同时测定MICs。MICs的变化以添加钙和镁后log(2)浓度增加的数量来表示。添加钙和镁后氨基糖苷类MICs的通常增加情况为：大肠杆菌为零个浓度，铜绿假单胞菌为一至六个浓度，大多数其他非发酵菌为一至两个浓度。庆大霉素对铜绿假单胞菌观察到最大的增加（五至六个浓度）。黏菌素MICs的通常增加情况为：大肠杆菌为零个浓度，铜绿假单胞菌为两个浓度，其他非发酵菌为一至两个浓度。四环素MICs的增加情况为：所有受试菌为一至五个浓度。羧苄青霉素MICs的通常增加情况为：大肠杆菌和铜绿假单胞菌为零个浓度，其他非发酵菌为零至两个浓度。这些观察结果表明，将穆勒-欣顿肉汤补充至推荐的钙和镁浓度对大肠杆菌的氨基糖苷类和黏菌素的MICs影响不大，但会增加大多数非发酵菌的MICs，增加大肠杆菌和所有非发酵菌的四环素MICs，对大肠杆菌和铜绿假单胞菌的羧苄青霉素MICs影响不大，但会增加除铜绿假单胞菌外的几种非发酵菌的MICs。
