Dorko E, Virágová S, Jautová J, Pilipcinec E, Danko J, Svický E, Tkáciková L
Department of Physiology, Faculty of Medicine, Safárik University, 040 66 Kosice, Slovakia.
Folia Microbiol (Praha). 2001;46(5):453-7. doi: 10.1007/BF02814438.
Electrophoretic karyotyping was used to compare DNA probes of yeasts isolated from blood of preterm neonates (n = 66) in a neonatal intensive care unit (NICU) and from the hands of healthy hospital personnel (n = 10). The yeasts were identified as Candida albicans using standard laboratory methods. DNA was extracted from yeasts and isolation of identical DNA strains from the pairs nurse-neonate suggested that one nurse transmitted one yeast strain by her hands to three neonates. Four neonates harbored two identical strains originating from two nurses, i.e. each nurse transmitted the same strain to two neonates. In the additional 7 cases transmission of 1 yeast strain by 1 nurse to 1 neonate was observed. Our data suggest that nonperinatal nosocomial transmission of C. albicans occurs in neonates, possibly via cross-contamination being transferred on hands of health care workers. The importance of careful hand washing of staff (health care workers) and other infection-control procedures (to prevent the nosocomial transmission of pathogens in the NICU environment) is emphasized.
采用电泳核型分析技术，比较从新生儿重症监护病房（NICU）的早产儿血液中分离出的酵母（n = 66）以及从健康医护人员手上分离出的酵母（n = 10）的DNA探针。使用标准实验室方法将酵母鉴定为白色念珠菌。从酵母中提取DNA，在护士 - 新生儿配对样本中分离出相同的DNA菌株，这表明一名护士通过她的手将一种酵母菌株传播给了三名新生儿。四名新生儿携带了来自两名护士的两种相同菌株，即每名护士将相同菌株传播给了两名新生儿。在另外7例中，观察到1名护士将1种酵母菌株传播给1名新生儿。我们的数据表明，白色念珠菌在新生儿中发生非围产期医院内传播，可能是通过医护人员手上的交叉污染传播。强调了工作人员（医护人员）认真洗手和其他感染控制程序（以防止NICU环境中医院内病原体传播）的重要性。
