Schweitzer D, Kolb A, Hammer M, Thamm E
Department of Experimental Ophthalmology, University of Jena, Germany.
Int Ophthalmol. 2001;23(4-6):399-404. doi: 10.1023/a:1014475219117.
The decay time is characteristic for several natural fluorophores. The determination of the decay time is independent of the fluorescence intensity. As a consequence, a short living weak fluorescence should be detectable also if it is covered by a intensive long-living fluorescence. As the decay time is influenced by the embedding matrix, information about the cellular stage might be possible. The laser scanning technique in combination with the time correlated single photon counting technique seems to be the optimal method for the discrimination of different fluorophores at the fundus according to the decay time. Fields of an equal decay time are presented as a tau mapping. An experimental set up was developed. Until now, only basic experiments were done on structured fluorescent tests, but under the conditions of the living eye. The results are promising. For the separate detection of the most important short life-time of 120 ps of A2E (excitation at 413 nm. emission 450-600 nm) as a putative precursor of lipofuscin in age-related macular degeneration [11], a light source for pulses in the range of about 50 ps is required.
衰变时间是几种天然荧光团的特征。衰变时间的测定与荧光强度无关。因此，即使短寿命的弱荧光被强的长寿命荧光所覆盖，也应该能够检测到。由于衰变时间受嵌入基质的影响，因此有可能获得有关细胞阶段的信息。激光扫描技术与时间相关单光子计数技术相结合，似乎是根据衰变时间区分眼底不同荧光团的最佳方法。具有相等衰变时间的区域以τ映射的形式呈现。开发了一种实验装置。到目前为止，仅在活体眼的条件下对结构化荧光测试进行了基础实验。结果很有前景。为了单独检测作为年龄相关性黄斑变性中脂褐素假定前体的A2E（在413nm激发，发射波长为450 - 600nm）的120ps的最重要短寿命，需要一个脉冲范围约为50ps的光源。
