AcrAB 外排系统：在克雷伯菌属中氟喹诺酮耐药性的表达及作用
AcrAB Efflux System: Expression and Contribution to Fluoroquinolone Resistance in Klebsiella spp.
作者信息
Mazzariol Annarita, Zuliani Jessica, Cornaglia Giuseppe, Rossolini Gian Maria, Fontana Roberta
机构信息
Dipartimento di Patologia, Sezione di Microbiologia, Università di Verona, Italy.
出版信息
Antimicrob Agents Chemother. 2002 Dec;46(12):3984-6. doi: 10.1128/AAC.46.12.3984-3986.2002.
Abstract
Seven Klebsiella pneumoniae and four Klebsiella oxytoca clinical isolates with different levels of resistance to ciprofloxacin were studied. Mutations in the topoisomerase genes were found in almost all strains, but the contribution of a multidrug efflux system homologous to AcrAB in Escherichia coli was also observed. Overexpression of this efflux system was demonstrated by immunoblotting with antibodies against E. coli AcrA.
摘要
对7株肺炎克雷伯菌和4株产酸克雷伯菌临床分离株进行了研究，这些菌株对环丙沙星具有不同程度的耐药性。几乎在所有菌株中都发现了拓扑异构酶基因的突变，但也观察到了与大肠杆菌中AcrAB同源的多药外排系统的作用。通过用抗大肠杆菌AcrA的抗体进行免疫印迹，证实了该外排系统的过表达。
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